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Appendix S1 Partial sequences of ATPα from 69 surveyed monarch butterflies in FASTA format. The intron used in analysis (see Methods) begins at position 100 and ends at position 188.

Appendix S2 Partial sequences of ATPα from seven surveyed queen butterflies in FASTA format. The intron used in analysis (see Methods) begins at position 46 and ends at position 126.

Appendix S3 Partial sequences for ATPα from additionally surveyed Nymphalidae butterflies in FASTA format (see Table  S1).

Appendix S4 Sequence for the H1–H2 associated intron from additionally surveyed Nymphalidae butterflies in FASTA format. This intron is located between positions 45 and 46 of the coding sequence (Appendix  S3).

Table S1 Detailed information for the species used in our population genetic and phylogenetic analyses of the Danainae subfamily.

Table S2 Summary of polymorphic and divergent sites in the intron of the H1–H2 extracellular domain of Na,K-ATPase.

Table S3 Taxa used in our phylogenetic study.

FilenameFormatSizeDescription
MEC_5379_sm_AppendixS1.txt17KSupporting info item
MEC_5379_sm_AppendixS2.txt2KSupporting info item
MEC_5379_sm_AppendixS3.txt1KSupporting info item
MEC_5379_sm_AppendixS4.txt0KSupporting info item
MEC_5379_sm_TableS1.docx20KSupporting info item
MEC_5379_sm_TableS2.xlsx49KSupporting info item
MEC_5379_sm_TableS3.xlsx30KSupporting info item

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