Both authors contributed equally to this work.
Genetic diversity of wild grapevine populations in Spain and their genetic relationships with cultivated grapevines
Article first published online: 12 DEC 2011
© 2011 Blackwell Publishing Ltd
Volume 21, Issue 4, pages 800–816, February 2012
How to Cite
DE ANDRÉS, M. T., BENITO, A., PÉREZ-RIVERA, G., OCETE, R., LOPEZ, M. A., GAFORIO, L., MUÑOZ, G., CABELLO, F., MARTÍNEZ ZAPATER, J. M. and ARROYO-GARCÍA, R. (2012), Genetic diversity of wild grapevine populations in Spain and their genetic relationships with cultivated grapevines. Molecular Ecology, 21: 800–816. doi: 10.1111/j.1365-294X.2011.05395.x
- Issue published online: 24 JAN 2012
- Article first published online: 12 DEC 2011
- Received 31 May 2010; revision received 10 October 2011; accepted 28 October 2011
- genetic diversity;
- genetic relationships;
- Vitis vinifera ssp. sylvestris;
- Vitis vinifera ssp. vinifera
The wild grapevine, Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi, considered as the ancestor of the cultivated grapevine, is native from Eurasia. In Spain, natural populations of V. vinifera ssp. sylvestris can still be found along river banks. In this work, we have performed a wide search of wild grapevine populations in Spain and characterized the amount and distribution of their genetic diversity using 25 nuclear SSR loci. We have also analysed the possible coexistence in the natural habitat of wild grapevines with naturalized grapevine cultivars and rootstocks. In this way, phenotypic and genetic analyses identified 19% of the collected samples as derived from cultivated genotypes, being either naturalized cultivars or hybrid genotypes derived from spontaneous crosses between wild and cultivated grapevines. The genetic diversity of wild grapevine populations was similar than that observed in the cultivated group. The molecular analysis showed that cultivated germplasm and wild germplasm are genetically divergent with low level of introgression. Using a model-based approach implemented in the software structure, we identified four genetic groups, with two of them fundamentally represented among cultivated genotypes and two among wild accessions. The analyses of genetic relationships between wild and cultivated grapevines could suggest a genetic contribution of wild accessions from Spain to current Western cultivars.