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Appendix S1 Palaeogeography of the NW European coastline.

Fig. S1 Palaeogeography of NW Europe during the Holocene. A-D Coastline development and ice margins beginning with the last glacial maximum (~20,000 years before present, 20ka BP) to approximately 8ka BP. From that age on, the Baltic Sea and the English Channel were permanently connected to the North Sea. Note that in Fig. S1B the part to the left of the broken white line reflects the situation at ~12ka BP when a huge meltwater lake was present in the area of the Irish and Celtic Sea, whereas the right part reflects the coastline at ~10.3ka BP. Abbreviations: BIS, British ice sheet; hd, Hurd Deep; ka BP, thousand years before present; SIS, Scandinavian ice sheet. The question mark (?) in Fig. S1A indicates the uncertain connection of BIS and SIS at the last glacial maximum. Arrows in Fig. S1D point to putative drainage sites of the Ancylus Lake.

Table S1 List of species and specimens analysed in this study with information on geography and collectors.

Table S2 Basic genetic properties of the sequenced specimens: haplotype and haplogroup assignment, presence of cryptic species (evidence only found in T. hyalina), fragment lengths, GenBank Accession nos. Sequence identifiers are mainly for internal use.

Table S3 Average uncorrected p-distances and standard deviation (in rectangular brackets) within (upper row) and between haplogroups of Turbanella cornuta calculated with mega 4 from the COI alignment.

Table S4 Average uncorrected p-distances and standard deviation (in rectangular brackets) within (upper row) and between haplogroups of Turbanella hyalina calculated with mega 4 from the COI alignment. Additionally, within-group average distance for Turbanella hyalina b-g and average distance between T. hyalina a and T. hyalina b-g is shown.

Table S5 K2P-corrected mean distances between morphospecies and molecular cryptic species (upper part). Major divergence events and half averaged K2P distance of all possible species pairs diverged from these nodes plus corresponding node age (in millon years) at an assumed COI substitution rate (K2P-corrected) of 1.23% per million years (lower part). [1]-[5]: distance values that were used to estimate depth of nodes # 1-5 (see Figure 3).

Table S6FST values between different geographic populations of T. cornuta.

FilenameFormatSizeDescription
MEC_5421_sm_TableS6.xls14KSupporting info item
MEC_5421_sm_TableS3.xls20KSupporting info item
MEC_5421_sm_TableS5.xls21KSupporting info item
MEC_5421_sm_TableS4.xls20KSupporting info item
MEC_5421_sm_AppendixS1.doc30KSupporting info item
MEC_5421_sm_FigS1.eps733KSupporting info item
MEC_5421_sm_TableS1.xls36KSupporting info item
MEC_5421_sm_TableS2.xls43KSupporting info item

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