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Numts help to reconstruct the demographic history of the ocellated lizard (Lacerta lepida) in a secondary contact zone

Authors

  • ANDREIA MIRALDO,

    1. School of Biological Sciences, University of East Anglia, Norwich NR4 7J, UK
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    • Present address: Metapopulation Research Group, University of Helsinki, Department of Biological and Environmental Sciences, P.O. Box 65, 00014 Helsinki, Finland.

  • GODFREY M. HEWITT,

    1. School of Biological Sciences, University of East Anglia, Norwich NR4 7J, UK
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  • PAUL H. DEAR,

    1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
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  • OCTAVIO S. PAULO,

    1. Centro de Biologia Ambiental, Departamento de Biologia Animal, Faculdade de Ciencias da Universidade de Lisboa, P-1749-016 Lisboa, Portugal
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  • BRENT C. EMERSON

    1. School of Biological Sciences, University of East Anglia, Norwich NR4 7J, UK
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    • Present address: Island Ecology and Evolution Research Group, IPNA-CSIC, C/Astrofísico Francisco Sánchez 3, 38206 La Laguna, Tenerife, Canary Islands, Spain.


Andreia Miraldo, Fax: +358 9 191 57694; E-mail: andreia.miraldo@helsinki.fi

Abstract

In northwestern Iberia, two largely allopatric Lacerta lepida mitochondrial lineages occur, L5 occurring to the south of Douro River and L3 to the north, with a zone of putative secondary contact in the region of the Douro River valley. Cytochrome b sequence chromatograms with polymorphisms at nucleotide sites diagnostic for the two lineages were detected in individuals in the region of the Douro River and further north within the range of L3. We show that these polymorphisms are caused by the presence of four different numts (I–IV) co-occurring with the L3 genome, together with low levels of heteroplasmy. Two of the numts (I and II) are similar to the mitochondrial genome of L5 but are quite divergent from the mitochondrial genome of L3 where they occur. We show that these numts are derived from the mitochondrial genome of L5 and were incorporated in L3 through hybridization at the time of secondary contact between the lineages. The additional incidence of these numts to the north of the putative contact zone is consistent with an earlier postglacial northward range expansion of L5, preceding that of L3. We show that genetic exchange between the lineages responsible for the origin of these numts in L3 after secondary contact occurred prior to, or coincident with, the northward expansion of L3. This study shows that, in the context of phylogeographic analysis, numts can provide evidence for past demographic events and can be useful tools for the reconstruction of complex evolutionary histories.

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