Appendix S1. Detailed methods and sampling information.

Data S1.GenBank accessions numbers.

Fig. S1 Distribution of estimated outcrossing rate.

Fig. S2 DAFS observed at B80 and expected one under neutrality for each population.

Fig. S3 Linear regressions between genetic diversity statistics (Ho, θW and πS) and outcrossing rate Tm for B160.

Table S1 Population diversity statistics for B80, B160 and reference loci in each population.

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