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The transcriptomic response to thermal stress is immediate, transient and potentiated by ultraviolet radiation in the sea anemone Anemonia viridis

Authors

  • A. MOYA,

    1. Université de Nice-Sophia-Antipolis, UMR7138 Systématique, Adaptation, Evolution, Valrose, BP71, 06108 Nice Cedex 02, France
    2. Université Pierre et Marie Curie & CNRS, UMR7138 Systématique, Adaptation, Evolution, 7 quai St Bernard, 75252 Paris Cedex 05, France
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  • P. GANOT,

    1. Université de Nice-Sophia-Antipolis, UMR7138 Systématique, Adaptation, Evolution, Valrose, BP71, 06108 Nice Cedex 02, France
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  • P. FURLA,

    1. Université de Nice-Sophia-Antipolis, UMR7138 Systématique, Adaptation, Evolution, Valrose, BP71, 06108 Nice Cedex 02, France
    2. Université Pierre et Marie Curie & CNRS, UMR7138 Systématique, Adaptation, Evolution, 7 quai St Bernard, 75252 Paris Cedex 05, France
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  • C. SABOURAULT

    1. Université de Nice-Sophia-Antipolis, UMR7138 Systématique, Adaptation, Evolution, Valrose, BP71, 06108 Nice Cedex 02, France
    2. Université Pierre et Marie Curie & CNRS, UMR7138 Systématique, Adaptation, Evolution, 7 quai St Bernard, 75252 Paris Cedex 05, France
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  • Present addresses: ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Australia and INSU-CNRS, UPMC, LOV, Observatoire Oceanologique de Villefranche, 06230 Villefranche-sur-Mer, France

  • These authors contributed equally to this work

Cecile Sabourault, Fax: 33 492 076 863; E-mail: cecile.sabourault@unice.fr

Abstract

Among the environmental threats to coral reef health, temperature and ultraviolet increases have been proposed as major agents, although the relative contribution of each in the cnidarian/zooxanthellae symbiosis breakdown has been poorly addressed. We have investigated the transcriptomic response to thermal stress, with and without ultraviolet radiation (UVR), in the symbiotic sea anemone Anemonia viridis. Using the Oligo2K A. viridis microarray, dedicated to genes potentially involved in the symbiosis interaction, we monitored the gene expression profiles after 1, 2 and 5 days of stresses that further lead to massive losses of zooxanthellae. Each stress showed a specific gene expression profile with very little overlap. We showed that the major response to thermal stress is immediate (24 h) but returns to the baseline gene expression profile after 2 days. UVR alone has little effect but potentiates thermal stress, as a second response at 5 days was observed when the two stresses were coupled. Several pathways were highlighted, such as mesoglea loosening, cell death and calcium homeostasis and described in more details. Finally, we showed that the dermatopontin gene family, potentially involved in collagen fibrillogenesis, issued from actinarian-specific duplication events, with one member preferentially expressed in the gastroderm and specifically responding to stress.

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