Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector

Authors

  • KARIMA ZOUACHE,

    1. Université de Lyon, F-69622 Lyon, France
    2. UMR CNRS 5557 Ecologie Microbienne, Universite Lyon 1, INRA USC 1193, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, F-69622 Lyon, France
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    • Present address: Institut Pasteur, Department of Virology, Arboviruses and Insect Vectors, Paris, France.

  • RORY J. MICHELLAND,

    1. Université de Lyon, F-69622 Lyon, France
    2. UMR CNRS 5557 Ecologie Microbienne, Universite Lyon 1, INRA USC 1193, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, F-69622 Lyon, France
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  • ANNA-BELLA FAILLOUX,

    1. Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, 25-28 rue du Dr Roux, F-75724 Paris Cedex 15, France
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  • GENEVIEVE L. GRUNDMANN,

    1. Université de Lyon, F-69622 Lyon, France
    2. UMR CNRS 5557 Ecologie Microbienne, Universite Lyon 1, INRA USC 1193, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, F-69622 Lyon, France
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  • PATRICK MAVINGUI

    1. Université de Lyon, F-69622 Lyon, France
    2. UMR CNRS 5557 Ecologie Microbienne, Universite Lyon 1, INRA USC 1193, VetAgro Sup, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, F-69622 Lyon, France
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Patrick Mavingui, Fax: +33 4 72 43 11 43; E-mail: patrick.mavingui@univ-lyon1.fr

Abstract

Mosquitoes transmit numerous arboviruses including dengue and chikungunya virus (CHIKV). In recent years, mosquito species Aedes albopictus has expanded in the Indian Ocean region and was the principal vector of chikungunya outbreaks in La Reunion and neighbouring islands in 2005 and 2006. Vector-associated bacteria have recently been found to interact with transmitted pathogens. For instance, Wolbachia modulates the replication of viruses or parasites. However, there has been no systematic evaluation of the diversity of the entire bacterial populations within mosquito individuals particularly in relation to virus invasion. Here, we investigated the effect of CHIKV infection on the whole bacterial community of Ae. albopictus. Taxonomic microarrays and quantitative PCR showed that members of Alpha- and Gammaproteobacteria phyla, as well as Bacteroidetes, responded to CHIKV infection. The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection, whereas the abundance of known insect endosymbionts like Wolbachia and Blattabacterium decreased. Our results clearly link the pathogen propagation with changes in the dynamics of the bacterial community, suggesting that cooperation or competition occurs within the host, which may in turn affect the mosquito traits like vector competence.

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