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Table S1 Databases for design of the fungi metabarcode, created by in silico PCR using ECOPCR.

Table S2 Fungi species used for primer testing in the laboratory.

Table S3 Bryophyte species used for primer testing in the laboratory.

Table S4 Specimen IDs and species names for Enchytraeidae samples that were used for primer testing in the laboratory.

Table S5 Coleoptera specimens used for primer testing in the laboratory.

Table S6 Taxonomic identities derived from the programs ecoTag and the Statistical Assignment Package (SAP) for cloned sequences.

Appendix S1 Supplementary material: Details of primer design and details of clone sequence identification.

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