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Table S1 Primer information.

Table S2 Comparison of models in BEAST.

Fig. S1 The ELW reference sequence from GenBank (accession no. NC_002646.1) (Miya & Nishida 2000) showing the position of the ribosomal genes, protein coding genes, control region and the positions of the primers used for the long-range PCRs.

Fig. S2 Maximum likelihood phylogeny made in MEGA v5 with 1000 bootstrap indicated above branches.

Fig. S3 Bayesian skyline plots (BSPs) of mitogenome data vs. a model of constant size.

Fig. S4 Bayesian skyline plots (BSPs) of mitogenome data vs. a model of constant size.

Fig. S5 Overview of parameters and model settings for the final simulation.

Fig. S6 Distributions of model parameters (current population effective size, Ne; expansion factor) using Approximate Bayesian Computation for the final simulations.

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