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Table S1 Web of Science search term combinations used for scanning the literature to assess multilocus species delimitation of closely related species

Table S2 Summary of rejected studies, grouped by reasons for rejection

Table S3 Characteristics of genetic markers used for delimitation of closely related species, grouped by clade or grade/cluster, with n representing the number of studies examined

Table S4 Accepted studies with corresponding data, sorted by study and locus

Table S5 All studies in literature survey, and results of sampling adequacy analysis, including number of specimens sampled per species (No. Spec./spp.), the estimated extent of geographic sampling (Sampling Extent), and global distribution of each species (Geog. Dist.)

Fig. S1 Number of separately used loci for each marker category and organism group showing: (A) fixed differences among species versus shared haplotypes, and (B) haplotype clades/clusters that are congruent versus non-congruent with species

Fig. S2 Sampling adequacy divided by marker type: (A) fixation, and (B) congruence indices categorized by the number of specimens sampled per species, and (C) fixation, and (D) congruence indices categorized by the extent of geographic sampling

Fig. S3 (A) Fixation and (B) congruence indices divided by both extent of geographic sampling and estimated global distribution

Appendix S1 Methods used in literature survey and analysis

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