Table S1 Summary statistics for thirteen microsatellite loci of Astroides calycularis

Table S2 Pairwise FST (lower left) and Dest (upper right) values.

Table S4 Results of the assignment tests for first-generation migrants of Astroides calycularis as the percentage of individuals from the sampling site assigned to every location (for locality codes see Table S1).

Table S5 Gene flow estimates among the clusters defined by STRUCTURE, based on a maximum likelihood approach of migration rates and population sizes (Ɵ = 4Neµ).

Fig. S1 Percentage of related pair-wise individuals.

Fig. S2 Plot of LnP(X|K) as a function of the number of clusters (K) across the 20 runs.

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