Fig. S1 Phylogeny and divergence time estimatesof Luculia inferred from 42 rbcL sequencesrepresenting species from all three subfamilies of Rubiaceae. Nodebars indicate the 95% HPD interval; Fossil-calibrated nodes areindicated by F1, F2 and F3, and it were constrained to 54, 45 and40 Mya, respectively. (Scale bar, Mya = millionyears ago).

Fig. S2 Phylogeny and divergence time estimatesof west-central and eastern lineages in Luculia pinceanainferred from the combined plastid dataset oftrnL-trnF and rpl20-rps12, with theprior age of the genus set to a normal distribution around the mean(5.462 Mya) estimated in the rbcL analysis. Node barsindicate the 95% HPD interval. (Scale bar, Mya = millionyears ago)

Fig. S3 The boundaries (red lines) detectedusing the BARRIER program based on 1000 bootstrapped matrices ofNei’s (1987) unbiased genetic distance (D) in a sampleof 25 populations of Luculia pinceana. Blue and black linesrepresent the Delaunay triangulation and the Voronoi tessellation,respectively. Two major genetic boundaries, with 96% (Barrier I)and 97% (Barrier II) mean bootstrap support, respectively,separated the central non-isoplethic populations from the easternand western isoplethic populations.

Table  S1 GenBank accession numbersfor rbcL sequences used to date the age ofLuculia.

Table S2 CpDNA sequence polymorphismsdetected in two intergenic spacer regions (trnL-trnFand rpl20-rps12) of Luculia pinceana identifying 10 haplotypes (HapA-J).

Data S1 Haplotype sequence.

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