Loss of floral polymorphism in heterostylous Luculia pinceana (Rubiaceae): a molecular phylogeographic perspective
Article first published online: 19 JUL 2012
© 2012 Blackwell Publishing Ltd
Volume 21, Issue 18, pages 4631–4645, September 2012
How to Cite
ZHOU, W., BARRETT, S. C. H., WANG, H. and LI, D.-Z. (2012), Loss of floral polymorphism in heterostylous Luculia pinceana (Rubiaceae): a molecular phylogeographic perspective. Molecular Ecology, 21: 4631–4645. doi: 10.1111/j.1365-294X.2012.05707.x
- Issue published online: 11 SEP 2012
- Article first published online: 19 JUL 2012
- Received 23 November 2011; revision received 24 May 2012; accepted 25 May 2012
Fig. S1 Phylogeny and divergence time estimatesof Luculia inferred from 42 rbcL sequencesrepresenting species from all three subfamilies of Rubiaceae. Nodebars indicate the 95% HPD interval; Fossil-calibrated nodes areindicated by F1, F2 and F3, and it were constrained to 54, 45 and40 Mya, respectively. (Scale bar, Mya = millionyears ago).
Fig. S2 Phylogeny and divergence time estimatesof west-central and eastern lineages in Luculia pinceanainferred from the combined plastid dataset oftrnL-trnF and rpl20-rps12, with theprior age of the genus set to a normal distribution around the mean(5.462 Mya) estimated in the rbcL analysis. Node barsindicate the 95% HPD interval. (Scale bar, Mya = millionyears ago)
Fig. S3 The boundaries (red lines) detectedusing the BARRIER program based on 1000 bootstrapped matrices ofNei’s (1987) unbiased genetic distance (D) in a sampleof 25 populations of Luculia pinceana. Blue and black linesrepresent the Delaunay triangulation and the Voronoi tessellation,respectively. Two major genetic boundaries, with 96% (Barrier I)and 97% (Barrier II) mean bootstrap support, respectively,separated the central non-isoplethic populations from the easternand western isoplethic populations.
Table S1 GenBank accession numbersfor rbcL sequences used to date the age ofLuculia.
Table S2 CpDNA sequence polymorphismsdetected in two intergenic spacer regions (trnL-trnFand rpl20-rps12) of Luculia pinceana identifying 10 haplotypes (HapA-J).
Data S1 Haplotype sequence.
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