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Fig. S1 Interaction plot of backtransformed LSM + and − the SE of the plasma corticosterone concentration in response to heat stress treatment.

Fig. S2 Range of gene expression in the liver for the HSP and antioxidant genes in response to heat stress, based on RNA-seq data.

Fig. S3 Catalase gene expression from the liver.

Fig. S4 MDS plot of antioxidant gene expression.

Fig. S5 Catalase nucleotide alignment of 10 unrelated individuals used in RNA-seq.

Fig. S6 SOD2 nucleotide alignment of 10 unrelated individuals used in RNA-seq.

Fig. S7 Allele frequenies differences among populations for SOD2 based on the 10 unrelated individuals with RNA-seq data.

Table S1 Table of characteristics between slow-living and fast-living ecotypes of garter snakes (Thamnophis elegans) from Lassen, Co. California. Modified from Schwartz & Bronikowski (2011).

Table S2 RNA-seq data

Table S3 Quantitative real-time PCR primers.

Table S4 Sample sizes for all analyses.

Table S5 Pairwise FST values among the four populations used in this study; all are significant at P < 0.05.

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