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Molecular cloning and characterization of the beta-amylase gene from Bacillus circulans
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1987.tb00531.x
1365-2958/asset/cover.gif?v=1&s=75254d9e3ae6a0f08085f34cc6e5bd65d8e3d52e "Molecular Microbiology")
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How to Cite
Siggens, K. W. (1987), Molecular cloning and characterization of the beta-amylase gene from Bacillus circulans. Molecular Microbiology, 1: 86–91. doi: 10.1111/j.1365-2958.1987.tb00531.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 12 March, 1987.
- Abstract
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Summary
A gene encoding the β-amylase of Bacillus circulans was isolated from a lambda library and sequenced. The structural gene consists of a 1725 bp open reading frame encoding a polypeptide with a predicted molecular wt of 62830 Daltons. Two active forms of the enzyme were found when the gene was expressed In E. coli. The larger 60 kD form was approximately 3 kD larger than the mature β-amylase secreted from B. circulans, suggesting that processing of this protein is different between the two species. The smaller 49 kD form is also present at a low level in B. circulans and may result from proteolytic cleavage. The enzyme has a temperature optimum of 50°C. Two other genes, one encoding an α-amylase and one a pullulanase, were also isolated from the lambda library.