The expression of mutant pilins in Pseudomonas aeruginosa: fifth position glutamate affects pilin methylation
Article first published online: 27 OCT 2006
Volume 2, Issue 4, pages 489–495, July 1988
How to Cite
Pasloske, B. L. and Paranchych, W. (1988), The expression of mutant pilins in Pseudomonas aeruginosa: fifth position glutamate affects pilin methylation. Molecular Microbiology, 2: 489–495. doi: 10.1111/j.1365-2958.1988.tb00055.x
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 18 March, 1988.
The expression within Pseudomonas aeruginosa PAO1 of three mutant pilin genes from P. aeruginosa PAK was studied to determine their effects on pilin stability, translocation into the membrane, leader peptide removal, and methylation of the mature N-terminal phenylalanine. The results revealed that a deletion of 4 or 8 amino acids within the immediate N-terminus of pilin had deleterious effects upon leader peptide cleavage. In addition, while the 4-amino-acid deletion did not affect pilin partitioning into the membrane, the 8-amino-acid deletion decreased the amount of pilin found within the membrane fraction. Of considerable interest was the finding that the mutation within the mature pitin of the glutamate at position 5 to a lysine did not prevent leader peptide removal but did inhibit the methylation of the N-terminal phenylalanine.