Expression of four virulence antigens of Shigella flexneri is positively regulated at the transcriptional level by the 30 kilo Dalton virF protein
Article first published online: 27 OCT 2006
Volume 2, Issue 5, pages 589–597, September 1988
How to Cite
Sakai, T., Sasakawa, C. and Yoshikawa, M. (1988), Expression of four virulence antigens of Shigella flexneri is positively regulated at the transcriptional level by the 30 kilo Dalton virF protein. Molecular Microbiology, 2: 589–597. doi: 10.1111/j.1365-2958.1988.tb00067.x
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 2 March, 1988; revised 7 May, 1988.
On the virulence plasmid of Shigella flexneri the virG region required for cell-to-cell spread of the bacteriumencodes a 130 kiloDalton (kD) antigen and Region-2essential for the bacterial invasion of epithelial cells encodes 57, 43 and 39 kD antigens. The expression of these four antigens is positively regulated by the 30 kD protein encoded by virF, whose nucleotide sequence had been determined and which was previously found to be essential for virulence. An approximately 3.8kilobase (kb) RNA transcript is found to be transcribed by the virG region and is positively regulated by the virF protein resulting in increased production of the 130 kD antigen. The virF sequence is conserved among all shigellae and enteroinvasive Escherichia coli.