Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system
Article first published online: 27 OCT 2006
Volume 2, Issue 6, pages 767–775, November 1988
How to Cite
Overduin, P., Boos, W. and Tommassen, J. (1988), Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system. Molecular Microbiology, 2: 767–775. doi: 10.1111/j.1365-2958.1988.tb00088.x
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 6 June, 1988; revised 21 July, 1988.
The nucleotide sequence of the ugp genes of Escherichia Coli K-12, which encode a phosphate-limitation inducible uptake system for sn-glycerol-3-phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a putative pho box, was detected by S1-nuclease mapping. The promoter is followed by four open reading frames, designated ugpB, A, E and C, which encode a periplasmic binding protein, two hydrophobic membrane proteins and a protein that is likely to couple energy to the transport system, respectively. The sequences of the proteins contain the characteristics of several other binding protein-dependent transport systems, but they seem to be particularly closely related to the maltose system.