Get access
Advertisement

Cloning and characterization of NSP1, a locus encoding a component of a CDC25-dependent, nutrient-responsive pathway in Saccharomyces cerevisiae

Authors

  • M. L. Tripp,

    1. Department of Biology, University of California, San Diego, La Jolla, California 92093, USA.
    Search for more papers by this author
    • Miller Brewing Company, Milwaukee, Wisconsin 53201. USA

  • R. A. Bouchard,

    1. Department of Biology, University of California, San Diego, La Jolla, California 92093, USA.
    Search for more papers by this author
    • Department of Biology, The College of Wooster, Wooster, Ohio 44691, USA.

  • R. Pinon

    Corresponding author
    1. Department of Biology, University of California, San Diego, La Jolla, California 92093, USA.
    • *For correspondence. Tel. (619) 534 2452; Fax: (619) 534 0053.

    Search for more papers by this author

Summary

The NSP1 gene in Saccharomyces cerevisiae has been identified by its ability, when expressed at high levels, to bypass the CDC25 requirement for growth. Sequence analysis of the cloned NSP1 locus suggests that the NSP1 product contains 269 amino acids and has a membrane-spanning domain at its carboxyl terminus. The NSP1 protein does not have sequence similarity to other known proteins, and is not related to the CDC25 protein, or to any of the previously described suppressors of CDC25 mutants. Phosphoprotein analysis of NSP1-suppressed cells indicates that the NSP1 product controls the phosphorylation of two 31 kD proteins whose phosphorylation and de-phosphorylation are strongly correlated with cell-cycle arrest and proliferation, respectively, and suggests that the NSP1 product is an important downstream element of a CDC25-dependent, nutrient-responsive, phosphorylation pathway.

Get access to the full text of this article

Ancillary