Squibb Institute for Medical Research, Princeton, New Jersey 08543m USA.
Activation of a cryptic crystal protein gene of Bacillus thuringiensis subspecies kurstaki by gene fusion and determination of the crystal protein insecticidal specificity
Article first published online: 27 OCT 2006
Volume 4, Issue 12, pages 2087–2094, December 1990
How to Cite
Dankocsik, C., Donovan, W. P. and Jany, C. S. (1990), Activation of a cryptic crystal protein gene of Bacillus thuringiensis subspecies kurstaki by gene fusion and determination of the crystal protein insecticidal specificity. Molecular Microbiology, 4: 2087–2094. doi: 10.1111/j.1365-2958.1990.tb00569.x
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 7 May, 1990.
DNA hybridization with the insecticidal crystal protein gene cryIIA (formerly cryBI) of Bacillus thuringiensis supspecies kurstaki has shown that subspecies kurstaki contains a cryIIA-related sequence in addition to the cryIIA gene (Donovan et al., 1988a). We have cloned the cryIIA-related sequence and have determined that the sequence, which has been designated cryIIB, is 89% identical to the cryIIA gene. Recombinant B. thuringiensis cells harbouring the cloned cryIIB gene produced very little CryIIB protein. A high level of production of the CryIIB protein was achieved by fusing the regulatory region of the cryIIIA crystal protein gene to the cryIIB gene. The CryIIB protein was found to be highly toxic to Lymantria dispar, Heliothis virescens and Trichoplusia ni, and was not toxic to Aedes aegypti.