Sequence of the peh gene of Erwinia carotovora: homology between Erwinia and plant enzymes

  1. J. C. D. Hinton1,†,
  2. D. R. Grll1,†,
  3. D. Lalo2,
  4. G. S. Plastow2,3,
  5. G. P. C. Salmond1,*

Article first published online: 27 OCT 2006

DOI: 10.1111/j.1365-2958.1990.tb00675.x

Issue

Molecular Microbiology

Molecular Microbiology

Volume 4, Issue 6, pages 1029–1036, June 1990

Additional Information

How to Cite

Hinton, J. C. D., Grll, D. R., Lalo, D., Plastow, G. S. and Salmond, G. P. C. (1990), Sequence of the peh gene of Erwinia carotovora: homology between Erwinia and plant enzymes. Molecular Microbiology, 4: 1029–1036. doi: 10.1111/j.1365-2958.1990.tb00675.x

Author Information

  1. 1

    Department of Biological Sciences, University of Warwick. Coventry CV4 7AL, UK.

  2. 2

    Leicester Biocentre, Leicester University, University Road, Leicester LE1 7RH, UK.

  3. 3

    Dalgety Plc, Group Research Laboratory, Station Road, Cambridge CB1 2JN, UK.

  1. †

    Imperial Cancer Research Fund Laboratories, University of Oxford, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, UK.

* *For correspondence. Tel. (0203) 523534; Fax (0203) 523701.

Publication History

  1. Issue published online: 27 OCT 2006
  2. Article first published online: 27 OCT 2006
  3. Received 8 November, 1989; revised 23 January, 1990.

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Summary

Polygalacturonase (Peh) and other pectolytic enzymes play a crucial role in the maceration of vegetables by soft rot Erwinia spp. We have sequenced the peh gene of Erwinia carotovora subsp. carotovora, and identified its product as a precursor of molecular weight 42639, and a mature protein of molecular weight 42200. A putative KdgR-binding site was identified in the region 5’to the peh gene. The Peh protein showed significant homology with Peh from tomato. In addition, we have found homologies between pectin methylesterase and pectate lyase from Erwinia and their counterparts in tomato. These homologies are described, and their significance discussed.

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