The ArsR protein is a trans-acting regulatory protein
Article first published online: 27 OCT 2006
Volume 5, Issue 6, pages 1331–1336, June 1991
How to Cite
Wu, J. and Rosen, B. P. (1991), The ArsR protein is a trans-acting regulatory protein. Molecular Microbiology, 5: 1331–1336. doi: 10.1111/j.1365-2958.1991.tb00779.x
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 2 January, 1991; revised 13 February, 1991.
The arsR gene encodes the regulatory protein of the plasmid-encoded arsenical resistance operon. A series of in-frame fusions was constructed between the C-terminally truncated arsR gene and the coding region for the mature form of β-lactamase (blaM). Fusions containing most of the arsR gene were still inducible by arsenicals. Fusions containing less than 102 residues of the 117-residue ArsR protein were constitutive. When a wild-type arsR gene was placed in trans, the constitutive constructs were again inducible. The results demonstrate that the ArsR protein is a trans-acting regulatory protein which controls its own expression.