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Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
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Transcriptional regulation of a Bacillus subtilis dipeptide transport operon
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1991.tb00815.x
Additional Information
How to Cite
Slack, F. J., Mueller, J. R., Strauch, M. A., Mathiopoulos, C. and Sonenshein, A. L. (1991), Transcriptional regulation of a Bacillus subtilis dipeptide transport operon. Molecular Microbiology, 5: 1915–1925. doi: 10.1111/j.1365-2958.1991.tb00815.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 13 March, 1991; revised 16 May, 1991.
- Abstract
- References
- Cited By
Summary
The Bacillus subtilis dciA operon, which encodes a dipeptide transport system, was induced rapidly by several conditions that caused the cells to enter stationary phase and initiate sporulation. The in vivo start point of transcription was mapped precisely and shown to correspond to a site of transcription initiation in vitro by the major vegetative form of RNA polymerase. Post-exponential expression was prevented by a mutation in the spo0A gene (whose product is a known regulator of early sporulation genes) but was restored in a spo0A abrB double mutant. This implicated AbrB, another known regulator, as a repressor of dciA. In fact, purified AbrB protein bound to a portion of the dciA promoter region, protecting it against DNase I digestion. Expression of dciA in growing cells was also repressed independently by glucose and by a mixture of amino acids; neither of these effects was mediated by AbrB.