These authors contributed equally to the studies reported here.
Location of the RNA-processing enzymes RNase III, RNase E and RNase P in the Eschenchia coli cell
Article first published online: 27 OCT 2006
Volume 5, Issue 7, pages 1801–1810, July 1991
How to Cite
Miczak, A., Srivastava, R. A. K. and Apirion, O. (1991), Location of the RNA-processing enzymes RNase III, RNase E and RNase P in the Eschenchia coli cell. Molecular Microbiology, 5: 1801–1810. doi: 10.1111/j.1365-2958.1991.tb01929.x
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 15 May, 1990; revised 11 February, 1991
Cells overexpressing the RNA-processing enzymes RNase III, RNase E and RNase P were fractionated into membrane and cytoplasm. The RNA-processing enzymes were associated with the membrane fraction. The membrane was further separated to inner and outer membrane and the three RNA-processing enzymes were found in the inner membrane fraction. By assaying for these enzymatic activities we showed that even in a normal wild-type strain of Escherichia coli these enzymes fractionate primarily with the membrane. The RNA part of RNase P is found in the cytosolic fraction of cells overexpressing this RNA, while the overexpressed RNase P protein sediments with the membrane fraction; this suggests that the RNase P protein anchors the RNA catalytic moiety of the enzyme to a larger entity. The implications of these findings for the cellular organization of the RNA-processing enzymes in the cell are discussed.