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Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1991.tb02114.x
1365-2958/asset/cover.gif?v=1&s=75254d9e3ae6a0f08085f34cc6e5bd65d8e3d52e "Molecular Microbiology")
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How to Cite
Cardy, D. L. N., Laidler, V., Salmond, G. P. C. and Murrell, J. C. (1991), Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b. Molecular Microbiology, 5: 335–342. doi: 10.1111/j.1365-2958.1991.tb02114.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 27 July, 1990; revised 21 September, 1990.
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Summary
The oxidation of methane to methanol in methano-trophic bacteria is catalysed by the enzyme methane monooxygenase (MMO). This multicomponent enzyme catalyses a range of oxidations including that of aliphatic and aromatic compounds and therefore has potential for commercial exploitation. This study details the molecular characterization of the soluble MMO (sMMO) genes from the Type II methanotroph Methylosinus trichosporium OB3b. The structural genes encoding the α, β and γ subunits of sMMO protein A and the structural gene encoding component B have been isolated and sequenced. These genes have been expressed and their products identified using an in vitro system. A comparative analysis of sMMO predicted sequences of M. trichosporium OB3b and the taxonomically related M. capsulatus (Bath) is also presented.