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Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1992.tb01768.x
1365-2958/asset/cover.gif?v=1&s=75254d9e3ae6a0f08085f34cc6e5bd65d8e3d52e "Molecular Microbiology")
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How to Cite
Clepet, C., Borne, F., Krishnapillai, V., Baird, C., Patte, J. C. and Cami, B. (1992), Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes. Molecular Microbiology, 6: 3109–3119. doi: 10.1111/j.1365-2958.1992.tb01768.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 13 December, 1991: revised 22 May, 1992; accepted 28 May, 1992.
- Abstract
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Summary
Three genes from Pseudomonas aeruginosa involved in threonine biosynthesis, hom, thrB and thrC, encoding homoserine dehydrogenase (HDH), homoserine kinase (HK) and threonine synthase (TS), respectively, have been cloned and sequenced. The hom and thrC genes lie at the thr locus of the P. aeruginosa chromosome map (31 min) and are likely to be organized in a bicistronic operon. The encoded proteins are quite similar to the Hom and TS proteins from other bacterial species. The thrB gene was located by pulsed-field gel electrophoresis experiments at 10 min on the chromosome map. The product of this gene does not share any similarity with other known ThrB proteins. No phenotype could be detected when the chromosomal thrB gene was inactivated by an insertion. Therefore the existence of isozymes for this activity is postulated. HDH activity was feedback inhibited by threonine; the expression of all three genes was constitutive. The overall organization of these three genes appears to differ from that in other bacterial species.