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Laboratory for Electron Microscopy, State University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands.
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Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1994.tb00301.x
Additional Information
How to Cite
Kiel, J. A. K. W., Boels, J. M., Beldman, G. and Venema, G. (1994), Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation. Molecular Microbiology, 11: 203–218. doi: 10.1111/j.1365-2958.1994.tb00301.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 23 July, 1993; revised and accepted 9 September 1993.
- Abstract
- References
- Cited By
Summary
Although it has never been reported that Bacillus subtilis is capable of accumulating glycogen, we have isolated a region from the chromosome of B. subtilis containing a glycogen operon. The operon is located directly downstream from trnB, which maps at 275 on the B. subtilis chromosome, it encodes five poly-peptides with extensive similarity to enzymes involved in glycogen and starch metabolism in both prokaryotes and eukaryotes. The operon is presumably expressed by an EσE-controlled promoter, which was previously identified downstream from trnB. We have observed glycogen biosynthesis in B. subtilis exclusively on media containing carbon sources that allow efficient sporulation. Sporulation-independent synthesis of glycogen occurred after integration of an EσA controlled promoter upstream of the operon.