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Regulation of transcription of the cell gene in Agaricus bisporus
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1994.tb00993.x
1365-2958/asset/cover.gif?v=1&s=75254d9e3ae6a0f08085f34cc6e5bd65d8e3d52e "Molecular Microbiology")
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How to Cite
Yagüe, E., Wood, D. A. and Thurston, C. F. (1994), Regulation of transcription of the cell gene in Agaricus bisporus. Molecular Microbiology, 12: 41–47. doi: 10.1111/j.1365-2958.1994.tb00993.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 21 October, 1993; revised 9 December, 1993: accepted 22 December. 1993.
- Abstract
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Summary
Northern analysis showed that accumulation of Agaricus bisporus cell mRNA was regulated by two independent mechanisms: (i) induction by cellulose; and (ii) repression by glucose and other sugars, Isolated A. bisporus nuclei were transcriptionally active. Nuclei Isolated from cellulose-grown mycelium synthesized six times more cell mRNA than nuclei from glucose-grown mycelium. The start point of transcription (tsp) was identified by primer extension and S1 nuclease analysis. Putative glucose-, and cAMP-responsive elements as well as regions with homology to promoter regions of other fungal cellulase genes were detected both upstream and downstream from the tsp of the cel1 gene.