1365-2958/asset/cover.gif?v=1&s=75254d9e3ae6a0f08085f34cc6e5bd65d8e3d52e "Molecular Microbiology")
Departmento de Biologia Celular, Laboratorio de Biologia Molecular, Universidade de Brasilia, Asa Norte, Brasilia DF CEP-70610, Brazil.
1365-2958/asset/olbannerleft.gif?v=1&s=5badf94c134fc8798f790efd4821c55ff95fc4cb)
Differential expression of multiple exo-cellobiohydrolase I-like genes in the lignin-degrading fungus Phanerochaete chrysosporium
Article first published online: 27 OCT 2006
DOI: 10.1111/j.1365-2958.1994.tb01010.x
Additional Information
How to Cite
Sims, P. F. G., Soares-Felipe, M. S., Wang, Q., Gent, M. E., Tempelaars, C. and Broda, P. (1994), Differential expression of multiple exo-cellobiohydrolase I-like genes in the lignin-degrading fungus Phanerochaete chrysosporium. Molecular Microbiology, 12: 209–216. doi: 10.1111/j.1365-2958.1994.tb01010.x
Publication History
- Issue published online: 27 OCT 2006
- Article first published online: 27 OCT 2006
- Received 22 March, 1993; revised 12 January, 1994; accepted 17 January, 1994.
- Abstract
- References
- Cited By
Summary
The genome of Phanerochaete chrysosporium strain ME446 contains multiple, non-allelic, cellobiohydrolase I (CSHI)-like sequences, at least two of which are expressed in a cellulose-dependent manner. Each of the expressed genes contains two identically positioned introns within its coding region. The lengths and sequences of these introns are different and one is not excised from all transcripts, raising the possibility that subtly different protein products may be expressed from a common gene. Introns are also present upstream of both genes but these differ in number and position, as well as sequence and length. Endoglucanase-like sequences could not be identified and it is suggested that variant CBHI-like proteins may provide endoglucanase activity in this fungus.