Region II of the Haemophilus influenzae Type b capsulation locus as involved in serotype-specific polysaccharide synthesis

  1. Johan Van Eldere1,*,
  2. Lisa Brophy2,
  3. Barbara Loynds2,
  4. Patrick Celts1,
  5. Ian Hancock3,
  6. Stephen Carman3,
  7. J. Simon Kroll4,
  8. E. Richard Moxon2

Article first published online: 27 OCT 2006

DOI: 10.1111/j.1365-2958.1995.tb02225.x

Issue

Molecular Microbiology

Molecular Microbiology

Volume 15, Issue 1, pages 107–118, January 1995

Additional Information

How to Cite

Van Eldere, J., Brophy, L., Loynds, B., Celts, P., Hancock, I., Carman, S., Kroll, J. S. and Moxon, E. R. (1995), Region II of the Haemophilus influenzae Type b capsulation locus as involved in serotype-specific polysaccharide synthesis. Molecular Microbiology, 15: 107–118. doi: 10.1111/j.1365-2958.1995.tb02225.x

Author Information

  1. 1

    Rega Institute for Medical Research, Catholic University of Leuven. Minderbroedersstraat 10, 3000 Leuven, Belgium.

  2. 2

    Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital Headington. Oxford OX3 9DU, UK.

  3. 3

    Department of Microbiology, University of Newcastle upon Tyne. Framlington Place, Newcastle upon Tyne NE2 4HH. UK.

  4. 4

    Molecular Infectious Diseases Group, Department of Paediatrics, St. Mary's Hospital Medical School, Imperial College, London W2 1PG, UK.

* *For correspondence. Tel. (16) 33 73 72; Fax (16)33 73 40.

Publication History

  1. Issue published online: 27 OCT 2006
  2. Article first published online: 27 OCT 2006
  3. Received 30 March, 1994; revised 1 September, 1994; accepted 5 September, 1994.

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Summary

The central (serotype-specific) Region II of the Haemophilus influenzae Type b capsulation locus cap is 8.3 kb long and contains a cluster of four genes. We show that these genes, designated orf1 to orf4, are involved in the biosynthetic steps required for the formation of the Type b capsular polysaccharide and that orf1 probably encodes a CDP-ribitolpyrophosphorylase. We present evidence that growth of polysaccharide chains takes place through the alternating addition of single sugar nucleotides.

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