National Food Biotechnology Centre, University College, Cork, Ireland.
Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t
Article first published online: 6 OCT 2006
Volume 19, Issue 6, pages 1343–1355, March 1996
How to Cite
van Sinderen, D., Karsens, H., Kok, J., Terpstra, P., Ruiters, M. H. J., Venema, G. and Nauta, A. (1996), Sequence analysis and molecular characterization of the temperate lactococcal bacteriophage r1t. Molecular Microbiology, 19: 1343–1355. doi: 10.1111/j.1365-2958.1996.tb02478.x
- Issue published online: 6 OCT 2006
- Article first published online: 6 OCT 2006
- Received 17 July, 1995; revised 15 November, 1995; accepted 20 November, 1995
The temperate lactococcal bacteriophage r1t was isolated from its lysogenic host and its genome was subjected to nucleotide sequence analysis. The linear r1t genome is composed of 33 350 bp and was shown to possess 3′ staggered cohesive ends. Fifty open reading frames (ORFs) were identified which are, probably, organized in a life-cycle-specific manner. Nucleotide sequence comparisons, N-terminal amino acid sequencing and functional analyses enabled the assignment of possible functions to a number of DNA sequences and ORFs. In this way, ORFs specifying regulatory proteins, proteins involved in DNA replication, structural proteins, a holin, a lysin, an integrase, and a dUTPase were putatively identified. One ORF seems to be contained within a self-splicing group I intron. In addition, the bacteriophage att site required for site-specific integration into the host chromosome was determined.