The sxa2-dependent inactivation of the P-factor mating pheromone in the fission yeast Schizosaccharomyces pombe

Authors

  • Graham Ladds,

    1. School of Biochemistry, The University of Birmingham, PO Box 363, Birmingham B15 2TT, UK.
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    • Department of Biologicalc Sciences, University of Warwick, Coventry CV4 7AL, UK

  • Erik Michael Rasmussen,

    1. School of Biochemistry, The University of Birmingham, PO Box 363, Birmingham B15 2TT, UK.
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    • Institute of Molecular Biology, Department of Genetics, University of Copenhagen, Oster Farimagsgade 2A, Copenhagen DK-1353, Denmark.

  • Tom Young,

    1. School of Biochemistry, The University of Birmingham, PO Box 363, Birmingham B15 2TT, UK.
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  • Olaf Nielsen,

    1. School of Biochemistry, The University of Birmingham, PO Box 363, Birmingham B15 2TT, UK.
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    • Institute of Molecular Biology, Department of Genetics, University of Copenhagen, Oster Farimagsgade 2A, Copenhagen DK-1353, Denmark.

  • John Davey

    Corresponding author
    • *For correspondence. Tel. (01203) 524 204; Fax (01203) 523 701.

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    • Department of Biologicalc Sciences, University of Warwick, Coventry CV4 7AL, UK


Summary

Haploid cells of the fission yeast Schizosaccharomyces pombe exist in one of two mating types, referred to as M and P. Conjugation occurs between cells of opposite mating type and is controlled by the reciprocal action of diffusible pheromones. Loss of function of the sxa2 gene in M cells causes hypersensitivity to the P-factor mating pheromone and a reduction in mating efficiency. Here we demonstrate the secretion of an sxa2-dependent carboxypeptidase that inactivates P-factor by removal of the C-terminal leucine residue.

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