Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan.
Induction of haemolytic activity in Escherichia coli by the slyA gene product
Article first published online: 6 OCT 2006
Volume 20, Issue 1, pages 191–199, April 1996
How to Cite
Oscarsson, J., Mizunoe, Y., Uhlin, B. E. and Haydon, D. J. (1996), Induction of haemolytic activity in Escherichia coli by the slyA gene product. Molecular Microbiology, 20: 191–199. doi: 10.1111/j.1365-2958.1996.tb02500.x
- Issue published online: 6 OCT 2006
- Article first published online: 6 OCT 2006
- Received 3 May, 1995; revised 11 December, 1995; accepted 22 December, 1995
The Salmonella typhimurium protein SlyAST, originally described as a cytolysin, shows sequence similarities to several known bacterial regulatory proteins. A homologue to the slyASt gene has been localised to min 37 of the Eschericia coli K-12 chromosome and has been designated slyAEC When introduced in trans on a plasmid, the slyAEC gene conferred a haemolytic phenotype on wild-type but not clyA-knockout strains of E. coli K-12. The clyA gene encodes a novel haemolysin that is not expressed by wild-type E. coli under tested laboratory conditions. Western and Northern blot analyses, and DNA-band-shift assays support a model whereby the SlyAEC protein activates clyA expression by binding to the clyA promoter region, thereby supporting the sequence similarity data in suggesting that SlyAST is a haemolysin activator rather than being a haemolysin per se.