Organizational characteristics and information content of an archaeal genome: 156kb of sequence from Sulfolobus solfataricus P2

  1. C. W. Sensen1,2,
  2. H.-P. Klenk3,†,
  3. R. K. Singh2,
  4. G. Allard4,
  5. C. C.-Y. Chan4,
  6. Q. Y. Liu4,
  7. S. L. Penny2,
  8. F. Young4,
  9. M. E. Schenk3,
  10. T. Gaasterland1,5,6,
  11. W. F. Doolittle1,3,
  12. M. A. Ragan1,2,*,
  13. R. L. Charlebois1,4

Article first published online: 6 OCT 2006

DOI: 10.1111/j.1365-2958.1996.tb02666.x

Issue

Molecular Microbiology

Molecular Microbiology

Volume 22, Issue 1, pages 175–191, October 1996

Additional Information

How to Cite

Sensen, C. W., Klenk, H.-P., Singh, R. K., Allard, G., Chan, C. C.-Y., Liu, Q. Y., Penny, S. L., Young, F., Schenk, M. E., Gaasterland, T., Doolittle, W. F., Ragan, M. A. and Charlebois, R. L. (1996), Organizational characteristics and information content of an archaeal genome: 156kb of sequence from Sulfolobus solfataricus P2. Molecular Microbiology, 22: 175–191. doi: 10.1111/j.1365-2958.1996.tb02666.x

Author Information

  1. 1

    Canadian Institute for Advanced Research, Program in Evolutionary Biology

  2. 2

    lnstitute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada.

  3. 3

    Department of Biochemistry, Sir Charles Tupper Medical Building, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada.

  4. 4

    Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario K1N 6N5, Canada.

  5. 5

    Mathematics and Computer Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA.

  6. 6

    Department of Computer Science, University of Chicago, 100 East 58th Street, Chicago, Illinois 60637, USA.

  1. †

    The Institute for Genomic Research, 9712 Medical Centre Drive, Rockville, Maryland 20850, USA.

* *For correspondence (address no. 2). E-mail: mark@imb.Lan.nrc.ca; Tel. (902) 426 1674; Fax (902) 426 9413.

Publication History

  1. Issue published online: 6 OCT 2006
  2. Article first published online: 6 OCT 2006
  3. Received 16 November, 1995; revised 16 July, 1996; accepted 22 July, 1996

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Summary

We have initiated a project to sequence the 3Mbp genome of the thermoacidophilic archaebacterium Sulfolobus solfataricus P2. Cosmids were selected from a provisional set of minimally overlapping clones, subcloned in pUC18, and sequenced using a hybrid (random plus directed) strategy to give two blocks of contiguous unique sequence, respectively, 100389 and 56105bp. These two contigs contain a total of 163 open reading frames (ORFs) in 26–29 putative operons; 56 ORFs could be identified with reasonable certainty. Clusters of ORFs potentially encode proteins of glycogen biosynthesis, oxidative decarboxylation of pyruvate, ATP-dependent transport across membranes, isoprenoid biosynthesis, protein synthesis, and ribosomes. Putative promoters occur upstream of most ORFs. Thirty per cent of the predicted strong and medium-strength promoters can initiate transcription at the start codon or within 10 nucleotides upstream, indicating a process of initial mRNA-ribosome contact unlike that of most eubacterial genes. A novel termination motif is proposed to account for 15 additional terminations. The two contigs differ in densities of ORFs, insertion elements and repeated sequences; together they contain two copies of the previously reported insertion sequence ISC 1217, five additional IS elements representing four novel types, four classes of long non-IS repeated sequences, and numerous short, perfect repeats.

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