Present address: Yale University School of Medicine, Section of Immunobiology, TAC660, 300 Cedar Street, New Haven, CT 06520, USA.
Mig-14 is an inner membrane-associated protein that promotes Salmonella typhimurium resistance to CRAMP, survival within activated macrophages and persistent infection
Article first published online: 9 DEC 2004
Volume 55, Issue 3, pages 954–972, February 2005
How to Cite
Brodsky, I. E., Ghori, N., Falkow, S. and Monack, D. (2005), Mig-14 is an inner membrane-associated protein that promotes Salmonella typhimurium resistance to CRAMP, survival within activated macrophages and persistent infection. Molecular Microbiology, 55: 954–972. doi: 10.1111/j.1365-2958.2004.04444.x
- Issue published online: 9 DEC 2004
- Article first published online: 9 DEC 2004
- Accepted 18 October, 2004.
The following supplementary material is available for this article: Fig. S1. Sequence of Mig-14 protein indicating peptides identified by mass-spectrometry. Underlined peptides were identified a single time, bold peptides were identified two independent times. Highlighted peptides were identified in outer membrane fraction, non-highlighted peptides were identified in inner membrane fraction. A complete analysis of the peptides identified during the sequencing is available at http://www.matrixscience.com/cgi/master_results.pl?file=../data/20040917/Fkncofan.dat. Fig. S2. FITC-CRAMP binding to additional S. typhimurium strains. Histograms of FITC fluorescence intensity of bacteria expressing DsRed treated with FITC-CRAMP and analysed as described in Fig. 6 and Experimental procedures. A. SL1344 B. mig-14 C. phoP D. SL1344 pMig-14. Similarly to mig-14, SL1344 pMig-14 shows a bimodal distribution of CRAMP staining, with approximately equal percentages of bacteria in the low and higher staining populations. E. trkA. Approximately 2-fold more trkA mutant bacteria than SL1344 are observed in the high staining population. F. sapAF. The histogram of FITC-CRAMP association with the sapAF mutant is virtually indistinguishable from that of SL1344.
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