Plasmids |

pIDN1 | IDM vector (Em^{R}) | Hamilton *et al*. (2001) |

pIDN2 | IDM vector (Em^{R}) | Hamilton *et al*. (2001) |

pIDN3 | IDM vector (Em^{R}) | Hamilton *et al*. (2001) |

pIDN4 | IDM vector (Em^{R}) | Hamilton *et al*. (2001) |

pNH9.9 | IDM vector precursor (Em^{R}) | Hamilton *et al*. (2001) |

pHSS6 | Cloning vector (Km^{R}) | Seifert *et al*. (1986) |

pKH9 | Precursor to complementation vector (Cm^{R}) | This work |

pKH35 | Complementation vector (Cm^{R}) | This work |

pJD1181 | GGI bp 20891–22930 in pNH9.9 | This work |

pHH13 | *Bam*HI-*Cla*I fragment (GGI bp 10739–11486) in pHSS6; *dsbC* clone | This work |

pHH16 | *dsbC*::*ermC*; pHH13 with *Acc*65I-cut *ermC* from pKS65 at *Bsr*GI site in *dsbC* | This work |

pHH20 | *Hind*III-*Xmn*I fragment of *traI* (GGI bp 4900–5323) in pIDN4 | This work |

pHH21 | *Sal*I-*Kpn*I fragment of *traN* (GGI bp 19663–20187) in pIDN4 | This work |

pHH23 | *Kpn*I-*Ssp*I fragment (GGI bp 56322–57358) in pIDN2; *parA* clone | This work |

pHH25 | Point mutation in *parA* Walker box A motif (K to Q) generated by overlapping PCR of pHH23 | This work |

pHH37 | PCR amplified, *Pac*I-, *Fse*I-digested *traH* in pKH9; *traH* complementation | This work |

pHH38 | PCR amplified, *Pac*I-, *Fse*I-digested *parA* in pKH9; *parA* complementation | This work |

pHH41 | *Not*I-*Eco*RI fragment of pHH13 in pKH35 (*Not*I-*Mun*I); *dsbC* complementation | This work |

pKS40 | Blunted PCR product of GGI bp 30514–30939 in pIDN3; *ydbA* sequence is in the opposite orientation as the *ermC* gene | This work |

pKS54 | *Eco*RV-*Bcl*I fragment of *traF* (GGI bp 21625–21952) in pIDN2 | This work |

pKS65 | *ermC* cassette vector | This work |

pKS67 | Chromosome walking product containing GGI bp 30514–33873 in pIDN3 | This work |

pKS69 | Chromosome walking product containing GGI bp 19685–21952 in pIDN2 | This work |

pKS72 | *Sal*I-*Kpn*I fragment of *traN* (GGI bp 19656–20180) in pIDN3 | This work |

pKS80 | Chromosome walking product containing GGI bp 13235–20187 | This work |

pKS83 | *Cla*I-*Hind*III fragment of *traC* (GGI bp 13235–13799) in pIDN3 | This work |

pKS88 | Chromosome walking product containing GGI bp 9377–13799 in pIDN3 | This work |

pKS94 | Chromosome walking product containing GGI bp 3395–10330 in pIDN2 | This work |

pKS100 | Chromosome walking product containing GGI bp 33247–42360 in pIDN1 | This work |

pKS124 | Chromosome walking product containing GGI bp 1–5412 and ∼ 4 kb upstream flanking DNA in pIDN2 | This work |

pKS130 | 839 bp, *Eco*RI-, *Ssp*I-cut PCR product containing a portion of the non-GGI gene *ung* in pIDN2 | This work |

pKS134 | Chromosome walking product containing GGI bp 47700–57358 and ∼1.1 kb downstream flanking DNA in pIDN2 | This work |

pKS139 | Chromosome walking product containing GGI bp 42268–44303 in pIDN1 | This work |

pKS140 | Chromosome walking product containing GGI bp 44294–47759 in pIDN1 | This work |

pARM3 | FA1090 *dif* and flanking DNA in pIDN2; generated by chromosome walking with pKS130 | This work |

pCBB1 | *Hpa*II-*Sau*3AI fragment from MS11A in pHSS7 with mTn*CmPhoA* in *exp1* | Dillard and Seifert (1997) |

pJS3 | *Psi*I fragment from pKS124 in pIDN2 | This work |

pWSP6 | *Hind*III-*Ssp*I fragment of pKS124 (*traD* fragment) in pIDN1 | This work |

pWSP7 | *traD* with 33 bp deletion of Walker box generated by overlapping PCR and digestion of pWSP6 | This work |

pKH54 | PCR amplified, *Hind*III-, *Spe*I-cut *traF* in pKH35; *traF* complementation | This work |

pKH55 | PCR amplified, *Hind*III-, *Spe*I-cut *traN* in pKH35; *traN* complementation | This work |

*N. gonorrhoeae* |

MS11A | Wild-type *N. gonorrhoeae* | Swanson *et al*. (1971) |

FA1090 | Laboratory strain of *N. gonorrhoeae* | H. S. Seifert |

1291 | Laboratory strain of *N. gonorrhoeae* | M. A. Apicella |

JC1 | Clinical isolate of *N. gonorrhoeae* | R. Hull |

RD5 | Laboratory strain of *N. gonorrhoeae* | R. S. Rosenthal |

F62 | Laboratory strain of *N. gonorrhoeae* | H. S. Seifert |

VP1 | Laboratory strain of *N. gonorrhoeae* | M. A. Apicella |

FA19 | Laboratory strain of *N. gonorrhoeae* | H. S. Seifert |

24-1 | Laboratory strain of *N. gonorrhoeae* | M. A. Apicella |

CV-1 | Laboratory strain of *N. gonorrhoeae* | H. S. Seifert |

CS7 | Laboratory strain of *N. gonorrhoeae* | H. S. Seifert |

UT 38794 | Low-passage clinical isolate of *N. gonorrhoeae* | W. L. Whittington |

UT 38884 | Low-passage clinical isolate of *N. gonorrhoeae* | W. L. Whittington |

UT 39260 | Low-passage clinical isolate of *N. gonorrhoeae* | W. L. Whittington |

NRL 8090 | Low-passage clinical isolate of *N. gonorrhoeae* | W. L. Whittington |

NRL 2025 | Low-passage clinical isolate of *N. gonorrhoeae* | P. A. Rice O’Brien *et al*. (1983) |

DGI 43 | Low-passage clinical isolate of *N. gonorrhoeae* | W. L. Whittington |

HH519 | MS11A transformed with pHH16; *dsbC*::*ermC* non-polar insertion mutant | This work |

HH522 | MS11A *exp1*::mTn*CmPhoA* constructed by transformation with pCBB-1 | This work |

HH532 | IDM of MS11A with pHH20; non-polar mutant of *traI* | This work |

HH535 | IDM of MS11A with pHH21; non-polar mutant of *traN* | This work |

HH540 | MS11A transformed with pHH25; *parA* point mutant | This work |

HH569 | HH519 transformed with pHH41; *dsbC* complement | This work |

HH570 | HH540 transformed with pHH38; *parA* complement | This work |

HH576 | KS16 transformed with pHH37; *traH* complement | This work |

HH577 | KS87 transformed with pKH54; *traF* complement | This work |

HH578 | HH535 transformed with pKH55; *traN* complement | This work |

KS16 | Non-polar IDM mutant of *traH* | Hamilton *et al*. (2001) |

KS57 | IDM of MS11A with pKS40; polar mutant of *ydbA* | This work |

KS60 | IDM of MS11A with pKS54; polar mutant of *traF* | This work |

KS75 | IDM of MS11A with pKS72; polar mutant of *traN* | This work |

KS87 | IDM of MS11A with pKS83; polar mutant of *traC* | This work |

KS131 | IDM of MS11A with pKS130; polar mutant of *ung* | This work |

ND500 | MS11AΔGGI constructed by transformation of HH522 with pARM3 | This work |

ND517 | ND500-Sp^{R} | This work |

ND518 | ND500 transformed with pJS3 | This work |

WSP7 | MS11A transformed with pWSP7; *traD* Walker box deletion | This work |