The PAS and HAMP domain superfamilies are signal transduction modules found in all kingdoms of life. The Aer receptor, which contains both domains, initiates rapid behavioural responses to oxygen (aerotaxis) and other electron acceptors, guiding Escherichia coli to niches where it can generate optimal cellular energy. We used intragenic complementation to investigate the signal transduction pathway from the Aer PAS domain to the signalling domain. These studies showed that the HAMP domain of one monomer in the Aer dimer stabilized FAD binding to the PAS domain of the cognate monomer. In contrast, the signal transduction pathway was intra-subunit, involving the PAS and signalling domains from the same monomer. The minimal requirements for signalling were investigated in heterodimers containing a full-length and truncated monomer. Either the PAS or signalling domains could be deleted from the non-signalling subunit of the heterodimer, but removing 16 residues from the C-terminus of the signalling subunit abolished aerotaxis. Although both HAMP domains were required for aerotaxis, signalling was not disrupted by missense mutations in the HAMP domain from the signalling subunit. Possible models for Aer signal transduction are compared.
The putative arrangement of domains in the dimeric Aer receptor is shown in Fig. 1. A membrane anchor separates the N-terminal PAS and F1 regions from the C-terminal HAMP and signalling domains. The signalling domain of Aer is homologous to the signalling domain of the chemoreceptors (Tsr, Tar, Trg and Tap), particularly at the highly conserved domain (HCD) (Bibikov et al., 1997; Rebbapragada et al., 1997). The histidine kinase CheA is coupled to the signalling domain by the CheW protein to form a stable ternary complex (Borkovich et al., 1989; McNally and Matsumura, 1991; Gegner et al., 1992). These interactions provide a point of convergence within the chemotaxis pathway, integrating signals from disparate chemoreceptors, and allowing co-ordinated control of the flagellar motors via a shared protein phosphorelay (as shown in Fig. 1).
The Tar HAMP domain from Salmonella enterica serovar Typhimurium consists of two α-helical amphipathic sequences, AS-1 and AS-2, connected by a region of undefined structure (Butler and Falke, 1998). The Aer HAMP domain appears to have a similar structure, except AS-2 contains additional hydrophobic residues and is predicted to be buried (Ma et al., 2005). FAD binding to the Aer-PAS domain requires an intact HAMP domain (Bibikov et al., 2000; Herrmann et al., 2004). This is supported by data showing that missense mutations in AS-2 eliminate FAD binding (Bibikov et al., 2000; Watts et al., 2004; Ma et al., 2005), and can be functionally suppressed by mutations in the PAS domain (Watts et al., 2004). Missense mutations in the proximal signalling region, on the other hand, disrupt signalling to the output domain, without influencing FAD binding, and are generally not resurrected by PAS suppressors (Bibikov et al., 2000; Watts et al., 2004; Ma et al., 2005). The cumulative evidence supports a model in which a contact domain between the HAMP AS-2 region and the Aer-PAS domain stabilizes FAD binding. It is likely that the aerotaxis signal is transmitted directly from the FAD-PAS domain to the HAMP or proximal signalling region, and then to the HCD.
Given the homodimeric nature of the Aer receptor (Ma et al., 2004), domain interactions that stabilize FAD binding or transduce the aerotaxis signal could feasibly occur in cis (within one subunit), or in trans (between cognate subunits). In the present study, we used intragenic complementation to address these questions. We identified inter-subunit (trans) interactions between the PAS and HAMP domains that stabilize FAD binding, but show that signal transduction from the N-terminal PAS input domain to the C-terminal output domain (the HCD) occurs in cis within a single Aer monomer. Further, we employed truncated Aer proteins to confirm these results, and identified the minimal Aer peptides required for aerotaxis signalling.
Aer dimers signal with one functional PAS and HAMP domain
The multidomain structure of the Aer protein makes it a candidate for intragenic complementation analysis. In previous genetic studies, aer mutations that abolished aerotaxis were identified in the coding regions of the PAS (codons 1–119), HAMP (207–253), proximal signalling (254–271), and signalling (272–506) domains (Bibikov et al., 2000; Repik et al., 2000; Watts et al., 2004; Ma et al., 2005). We selected mutations from those studies (Fig. 2) and, using compatible plasmids, coexpressed two Aer proteins with missense mutations in different domains. Mutant Aer proteins were expressed in the aerotaxis- and recombination-deficient background of BT3400 (aer, tsr, recA). The expectation was that mutant Aer subunits would combine to form inactive homodimers, as well as a heterodimer containing one subunit from each mutant Aer protein. Transformants were screened on succinate semi-soft agar to identify heterodimers that recovered aerotaxis through intragenic complementation.
The utility of this technique was evident from initial experiments in which Aer-PAS and Aer-HAMP mutant proteins were coexpressed from compatible pACYC184- and pTrc99A-derived constructs. We designate mutant Aer proteins with an M1 (monomer 1) subscript if they are expressed from a pACYC184-derived plasmid, and an M2 (monomer 2) subscript if they are expressed from a pTrc99A-derived plasmid. Both M1 and M2 constructs were under the control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible, ptrc promoter. Heterodimers of the coexpressed Aer-PASM1 and Aer-HAMPM2 mutants recovered aerotaxis on succinate semi-soft agar plates, indicating Aer can function with one wild-type (WT) PAS and one WT HAMP sequence per dimer. For example, when the PAS mutant Aer-N34DM1 was coexpressed with the HAMP mutant Aer-V230DM2, or Aer-D68CM1 was coexpressed with Aer-G240RM2, aerotaxis was restored to BT3400 (Fig. 3). Following the success of these initial experiments, we designed a series of specific PAS, HAMP, signalling domain and truncation mutants to investigate the mechanics of signalling in Aer by intragenic complementation.
The HAMP domain stabilizes FAD binding to the cognate PAS domain
Each of the Aer-PASM1 + Aer-HAMPM2 heterodimers were aerotactic, promoting ring formation on succinate semi-soft agar (Table 1, Fig. 3). We interpret this to mean that the Aer-HAMPM2 mutants prevent FAD binding to the cognate Aer-PASM1 domain but not to the PASM2 domain. Otherwise neither PAS domain in the heterodimer would be functional. This was particularly evident where both the PAS and HAMP mutants disrupted FAD binding, because neither PAS domain in the heterodimer would bind FAD. Therefore, PAS/HAMP interactions must be inter-subunit, resulting in aerotactic heterodimers containing one functional PAS domain, and a functional cognate HAMP domain. The heterodimers continued to support aerotaxis when more severe lesions were introduced into the HAMP domain (V230D/G240R, V230D/D237G and V230D/Q248R), although some of the responses were weaker (Table S1).
The complementation experiments were repeated using FAD– mutants with mutations at the C-terminus of the HAMP domain [L251P and C253R (Watts et al., 2004)] and FAD+ mutants with mutations in the proximal signalling region [L256R and D259H (Ma et al., 2005)] (Fig. 2). Overall, these mutants were less successful than the previous HAMP mutants in complementing the various Aer-PASM1 mutants; resultant swarms ranged from non-functional to partially functional, and aerotactic (–, +/– and + respectively, in Table 1; also see Fig. 3). Partially aerotactic heterodimer swarms were smaller than their aerotactic counterparts, and contained less-defined rings (see example in Fig. 3). It is possible that these residues (251–259) occur at a critical juncture, and play different roles in signalling and in stabilizing FAD binding than the preceding HAMP residues (230–248). Poor complementation could also reflect lower cellular levels of the Aer-PASM1 mutants expressed from the pACYC184 plasmid (Fig. S1), which has a lower copy number than pTrc99A. We previously reported that Aer mutants are often found at lower concentrations in the cell, even though they have normal mRNA levels (Ma et al., 2005). This suggests a higher rate of post-translational turnover. The success of Aer-N34DM1 in complementing mutations in this region (residues 230–259, Table 1) may be related to its higher expression compared with the other Aer-PASM1 mutants (Fig. S1).
The only M2 mutant not efficiently complemented by Aer-N34DM1 was Aer-C253RM2 (Table 1). This finding was unexpected because N34D is an allele-specific suppressor for C253R (Watts et al., 2004), and expression differences do not appear to explain the result. As an alternative explanation for poor complementation, we tested for phenotypic dominance by coexpressing the C-terminal HAMPM2 and proximal signallingM2 mutants with a WT AerM1 construct where WT expression was induced from a tightly regulated pnahG promoter. Titrations were performed with sodium salicylate (the WT M1 construct) and IPTG (M2 mutant constructs) to determine induction levels resulting in an M1/M2 expression ratio of approximately 1:1. Under these conditions, Aer-C253R and Aer-D259H were dominant over WT Aer and prevented aerotaxis, while Aer-L251P and Aer-L256R were recessive to WT function (Fig. S2). The dominant behaviour of Aer-C253R and Aer-D259H help explain the inferior complementation observed using these mutants (Table 1). In addition, dominant Aer-C253R homodimers could be expected to inhibit signalling by Aer-N34DM1 + Aer-C253RM2 heterodimers, resulting in the partially aerotactic phenotype observed.
Aer signals through one subunit
After finding that PAS–HAMP interactions stabilize FAD binding asymmetrically, we wanted to determine whether signals sensed by the PAS domain are transduced to the signalling region of the same monomer, or to the cognate monomer. To distinguish between these possibilities, an M341T missense mutation (Bibikov et al., 2000) was added to the signalling domain. As shown in Fig. 4, heterodimers were only aerotactic when functional PAS and signalling domains were located on the same monomer (Aer-N34D/M341TM1 + Aer-Q248RM2), arguing for signalling within one subunit of the Aer dimer. An Aer-Q248RM1 + Aer-M341TM2 heterodimer was similarly aerotactic (Fig. 5). The non-aerotactic Aer-N34DM1 + Aer-Q248R/M341TM2 heterodimer, which simulated an inter-subunit signalling pathway (Fig. 4), was created from constructs with comparable, stable, expression levels. Therefore expression differences did not contribute to the loss of aerotaxis when M341T was swapped from the N34DM1 to the Q248RM2 monomer (data not shown).
To confirm the intra-subunit signalling pathway hypothesis, we created C-terminal Aer truncations and coexpressed them with full-length Aer-Q248RM1 (1–506). The idea was to disrupt FAD binding to the PASM2 domain of each truncated monomer, so that the M1 subunit would become the signalling monomer. Truncations of the Aer C-terminus were made at residues 490 and 285 in the signalling domain (Δ491–506 and Δ286–506 respectively), at residues 260 and 256 in the proximal signalling domain (Δ261–506 and Δ257–506 respectively), at residue 253 on the HAMP/proximal signalling domain boundary (Δ254–506), and within the HAMP domain at residue 250 (Δ251–506) (Fig. 2). None of these truncations were aerotactic as homodimers (Fig. 5A).
Truncations that deleted the terminal 16 residues (Aer[1–490]M2), or most of the signalling domain (Aer[1–285]M2), from the non-signalling subunit, did not disrupt aerotaxis of heterodimers with Aer-Q248RM1 (Fig. 5). This indicates that the M2 signalling domain is not necessary for aerotaxis, and the M1 signalling domain is sufficient. The smaller swarm size seen with the Aer[1–285]M2 heterodimer (Fig. 5) may be due to the loss of CheA/W interaction sites from Aer[1–285], or due to high expression of the mutant protein (approximately threefold greater than WT AerM2). Aer[1–490]M2 contains CheA/W interaction sites and had similar expression to WT AerM2. Two complete HAMP domains were required for aerotaxis in the truncation heterodimers, because function was lost with truncations between residues 260 and 256. The Aer-Q248RM1 + Aer[1–260]M2 heterodimer retained some aerotactic capacity, whereas the Aer[1–256]M2, Aer[1–253]M2 and Aer[1–250]M2 truncation heterodimers were non-aerotactic (Fig. 5). This was not related to expression differences, because Aer[1–260]M2 and Aer[1–256]M2 expression was similar to Aer-WTM2.
To determine if residues deleted from the signalling monomer would affect aerotaxis, Aer[1–490]M2 was coexpressed with full-length Aer-N34DM1. FAD should bind to the Aer[1–490] PASM2 domain of this heterodimer, and the truncated M2 subunit becomes the putative signalling monomer. Deletion of just 16 residues from the C-terminus of the signalling monomer abolished function in the Aer-N34DM1 + Aer[1–490]M2 heterodimer (Table S1). This is in contrast to deletion of the non-signalling Aer subunit in the Aer-Q248RM1 + Aer-truncationM2 heterodimers above, where approximately 240 residues could be removed, and aerotaxis retained.
Sensing with one PAS domain
When the Aer-PASM1 mutants (N34D, G42C, D68C, Y93H and M21D) were coexpressed with Aer-N34DM2, we observed no complementation between PAS mutations, consistent with a requirement for one functional PAS domain per dimer (Table S1). To confirm that aerotaxis requires only one PAS domain, N-terminal Aer-PASM2 truncations were constructed (Fig. 2) and coexpressed with Aer-Q248RM1(Fig. 6). Heterodimers with Aer[120–506]M2 (with the PASM2 domain deleted), or Aer[120–490]M2 (with the PASM2 domain and part of the C-terminalM2 signalling domain deleted), produced aerotactic swarms, although they were smaller than most other aerotactic heterodimer swarms (see enlargement in Fig. 6B). Aer can therefore sense and signal with one PAS domain per dimer, extending our previous findings in which aerotaxis was supported with one functional and one inactive PAS domain (Table 1 and Fig. 3). The PAS truncation heterodimers also provide evidence that Q248RM1 disrupts FAD binding to the cognate PASM2 domain, as posited previously. Aer-Q248RM1 could not disrupt FAD binding to the PASM1 domain in cis, because it was the only PAS domain present, and the heterodimers retained aerotactic function.
An Aer construct lacking both the PAS and F1 domains (Aer[165–506]M2) was also assayed for its ability to support aerotaxis in a heterodimer with Aer-Q248RM1. Although Aer[165–506]M2 had similar expression levels to Aer[120–506]M2, a heterodimer between Aer[165–506]M2 and Aer-Q248RM1 was non-aerotactic (Fig. 6). One interpretation is that the F1 domain is needed to stabilize FAD binding (Bibikov et al., 2000) through inter-subunit interactions (in a similar manner to the HAMP domain). Alternatively, the F1 region may be necessary for proper folding or for correct membrane insertion. An Aer peptide lacking the PAS and C-terminal signalling domains (Aer[120–285]M2) was also non-aerotactic when coexpressed with Aer-Q248RM1 (Table S1). Although not tested, this short Aer peptide may not fold correctly, or dimerize with full-length Aer.
The presence of both a PAS and HAMP domain in the Aer receptor enabled us to investigate PAS/HAMP interrelationships. We determined that FAD binding was stabilized by PAS and HAMP domains from cognate subunits (Fig. 3 and Table 1), but that the signal transduction pathway proceeded from the PAS domain to the signalling domain of the same subunit (Figs 4–6, and Table S1). The asymmetric role of two Aer subunits in FAD binding was demonstrated by intragenic complementation between PAS mutants and HAMP mutants (residues 230–248 in AS-2, Table 1). Substitutions in residues 251–259 showed incomplete complementation, raising the possibility that these residues have a different role from residues 230–248. We considered an alternative possibility that the WT HAMP domain exerted its effects indirectly by correcting malformations in the mutant HAMP domain, which itself could resurrect FAD binding and aerotaxis. However, this is unlikely, given the range of HAMP defects that were compensated in heterodimers. Although it was reported that symmetric signalling through both HAMP domains is necessary in Tar/EnvZ chimeras (Zhu and Inouye, 2004), the Aer protein required only one WT HAMP sequence for signalling.
Previous studies support a model in which the native fold of the Aer-PAS domain is stabilized by protein/protein interactions with the HAMP domain (Herrmann et al., 2004; Watts et al., 2004). The results of the present study suggest the PAS/HAMP contact domain is between cognate Aer subunits (see Fig. 7A). Although there is a crossover between the PAS and HAMP domains, the signalling pathway does not continue down the cognate subunit (Figs 4–6). This finding raises questions about the role of the HAMP domain in signalling. We propose three possible models for signalling in Aer (Fig. 7B–D). In the first model (Fig. 7B), the signalling pathway from a PAS domain is via the HAMP domain on the same monomer. The model assumes that signalling though the HAMP domain is not disrupted by HAMP mutations such as those in Table 1. In the second model (Fig. 7C), signalling crosses over from the PASM1 domain to the cognate HAMPM2 domain, and returns to the signallingM1 domain. The cognate HAMP domain had a WT sequence in all aerotactic heterodimers tested in this study. In the third model (Fig. 7D), the signal is transmitted directly from the PAS domain to the proximal signalling domain of the same subunit. By bypassing either HAMP domain, potential difficulties in propagating signals despite HAMP defects are avoided. Irrespective of a specific model, PAS signals are apparently transduced to the proximal signalling domain before residue 260, because Aer/Tsr chimeras incorporating the first 260 residues from Aer are fully functional (Repik et al., 2000). The model may also account for the different complementation pattern observed for residues 251–259 in Table 1.
Heterodimers between full-length and truncated Aer monomers were used to investigate the minimum requirements for the non-signalling subunit. Deletion of the PAS (Fig. 6) or signalling (Fig. 5) domain did not prevent aerotaxis in heterodimers. However, deletion of part of the HAMP domain abolished signalling (Fig. 5), probably by disrupting FAD binding to the signalling PAS domain. The F1 domain (Fig. 1) was also required for signalling (Fig. 6). Similar C-terminal truncations of the non-signalling subunit in the Tar chemoreceptor have been reported, except that part of the HAMP domain could also be deleted without inactivating signalling (Gardina and Manson, 1996; Tatsuno et al., 1996). As chemoreceptors form trimers of dimers (Kim et al., 1999; 2002; Ames et al., 2002), receptors with a truncated signalling domain likely create dimeric signalling domains by swapping domains (Gardina and Manson, 1996) within a trimer of dimers.
The full-length signalling subunit was required for aerotaxis by Aer. Deletion of 16 residues from the C-terminus abolished signalling by both homodimers and heterodimers (Table S1). This differs from Tar, which activates CheA normally when 35–37 residues are removed from the C-terminus (Russo and Koshland, 1983; Francis et al., 2004). However, the C-terminus of Tar extends 39 residues beyond that of Aer. By analogy to other chemoreceptors (Le Moual and Koshland, 1996; Kim et al., 1999), it is likely that the C-terminal segment of Aer forms an anti-parallel coiled-coil with the proximal signalling domain. If so, C-terminal truncations could destabilize the proximal signalling region and abolish aerotaxis.
In this study we established that the HAMP domain stabilizes FAD binding asymmetrically to the cognate PAS domain, but that the signalling pathway proceeds in cis, within one Aer subunit. The role of the HAMP and proximal signalling domains is intriguing. Although both HAMP domains are required for function, missense mutations in the HAMP domain are tolerated by the signalling subunit. Further studies are required to determine whether the Aer-HAMP domain has a direct role in signalling, in addition to its role in stabilizing the native fold of the cognate PAS domain.
Parental plasmids included pACYC184 (Chang and Cohen, 1978), pTrc99A (Pharmacia, discontinued) and pProEX™HTa (Invitrogen, Carlsbad, CA). The pACYC184 plasmid contains a p15A replication origin, allowing coexpression of genes with pTrc99A or pProEX™HTa plasmids, both of which are members of the ColE1 compatibility group. Relevant strain and plasmid properties are given in Table S2.
Construction of Aer mutants
A pTrc99A derivative expressing WT aer under the control of an IPTG-inducible ptrc promoter, pKW1, and several pKW1 derivatives expressing Aer missense mutants were constructed previously (Watts et al., 2004). Constructs expressing Aer-M341T (Bibikov et al., 2000) or Aer-L256R were constructed by site-directed mutagenesis using pKW1 as template, PfuTurbo® (Stratagene, La Jolla, CA), and primers specific for the introduction of each mutation. Primers were designed as outlined in the QuikChange® site-directed mutagenesis kit (Stratagene). Aer-L256R was identified as a null FAD+ aerotaxis mutant by site-specific mutagenesis of residues in proximity to C253. To create the double HAMP mutants V230D/D237G, V230D/G240R and V230D/Q248R, plasmids pKW2 (Aer-D237G), pKW3 (Aer-G240R) and pKW11 (Aer-Q248R) were amplified with PfuTurbo® using V230D-specific primers. A construct expressing Aer-Q248R/M341T was made by site-directed mutagenesis using pKW11 (Aer-Q248R) and M341T-specific primers.
Constructs expressing Aer-D68C (Repik et al., 2000), Aer-G42C (Repik et al., 2000) and Aer-Y93H (Bibikov et al., 2000) were made by site-directed mutagenesis using pKW1 as template with mutation-specific primers. To transfer DNA encoding Aer-D68C (pKW86) and Aer-N34D (pKW74) into pACYC184, the plasmid region encompassing the ptrc promoter, mutated aer, and lacIq was removed with SphI, treated with Klenow, and ligated into the ScaI site of pACYC184. The pACYC184/aer mutant pKW89 (Aer-N34D) was then used as a template to clone different aer mutants into pACYC184. Plasmids encoding Aer-M21D (pKS1), Aer-Q248R (pKW11), Aer-G42C (pKW87) and Aer-Y93H (pKW88) were digested with HpaI and PstI to remove a 2 kb fragment comprising aer and a 500 bp fragment of pTrc99A. These fragments were ligated into pKW89 with the corresponding HpaI–PstI fragment (including the region encoding N34D) removed. A construct expressing Aer-N34D/M341T from pACYC184 was made by digesting pKW89 with KpnI and PstI, and swapping the 1.2 kb WT aer fragment with the KpnI–PstI fragment from pKW81 (Aer-M341T).
C-terminal Aer truncations were created by polymerase chain reaction (PCR) of pKW1 using a primer complementary to the SphI site at pTrc99A nucleotide 2962 (pTrcSphIF). This was paired with individual primers containing stop codons after Aer residues 490, 285, 260, 256, 253 and 250, followed by an engineered SphI site (AerSphIR primers). PCR products were digested with SphI and ligated to the 2.64 kb SphI fragment of pTrc99A.
N-terminal Aer truncations, Δ1–119 and Δ1–164, were created by PCR of pKW1 using primers complementary to the NcoI site at pTrc99A nucleotide 265 (Aer120NcoIF and Aer165NcoIF), each paired with a primer containing an engineered SalI site (Aer506SalIR). The Aer120NcoIF and Aer165NcoIF primers incorporated a start codon, followed by the codon for residue 120 or 165, while the Aer506SalIR primer included the codon for residue 506 followed by the normal stop codon of Aer. PCR products were digested with NcoI and SalI and ligated to the 4.14 kb NcoI–SalI fragment of pTrc99A. An Aer Δ[1–119/491–506] construct was made by the same method using Aer120NcoIF and Aer490SalIR primers. An Aer Δ1–119/Δ286–506 construct was made by a similar method using Aer120NcoIF and Aer285SalIR primers, except the PCR product was ligated into the NcoI and SalI sites of pProEX™Hta, which encodes an N-terminal His6x tag. Primer sequences for the construction of all truncations are provided in Table S2. The expected mutations and/or truncations were confirmed for all constructs by DNA sequencing. The expression of full-length and truncated Aer proteins were verified by chemiluminescent Western blotting using Anti-Aer2−166 antisera (Repik et al., 2000), except for Aer[120–285], where expression was verified using an INDIA™ HisProbe (Pierce, Rockford, IL).
BT3400 was transformed with individual pTrc99A/aer or pACYC184/aer derivatives to create Aer homodimers, or simultaneously with both plasmids to create Aer heterodimers. Transformants were selected on LB thiamine (0.5 µg ml−1) agar plates containing 100 µg ml−1 ampicillin (pTrc99A constructs), 15 µg ml−1 tetracycline (pACYC184 constructs), or both ampicillin and tetracycline (pTrc99A and pACYC184 constructs). To analyse aerotaxis phenotypes, individual BT3400 transformant colonies were transferred with sterile toothpicks into 30 mM succinate semi-soft agar swarm plates containing 0.28% agar and either 50 µg ml−1 ampicillin, 7.5 µg ml−1 tetracycline, or both antibiotics. A minimum of four clones were tested on semi-soft agar, and each experiment was repeated two to four times. Initially, we used BT3400/pKW1 as a positive aerotaxis control, and BT3400/pTrc99A as a negative aerotaxis control in swarm plates containing ampicillin. Heterodimers containing Aer-N34D and Aer-V230D were used as positive aerotaxis controls on all swarm plates after initial experiments with these mutants showed intragenic complementation. Swarm plates were incubated for 24–48 h at 30°C and analyses were made at 12, 24 and 48 h. Appropriate homodimer controls were carried out simultaneously with every heterodimer experiment.
To test for dominant behaviour, mutant Aer constructs were co-transformed with pDS7, which expresses WT Aer from pACYC184 using a tightly regulated sodium salicylate-inducible promoter (nahG). The pDS7 plasmid was constructed from pKG117 (kindly provided by K. Gosink) by adding the tetracycline-resistance gene back to the pACYC184 portion of the plasmid with ClaI. Dominance tests were performed the same way as the heterodimer assays outlined above, except 0.5 µM or 1 µM sodium salicylate, and IPTG concentrations between 0 and 0.6 mM were included in the swarm plates in a series of titrations. Induction levels producing approximately 1:1 expression ratios were determined by Western blot.
We are grateful to Sheena Fry and Nathan Abraham for technical assistance, to Asharie Campbell for constructing pAJC1, to Daniel Salcedo for constructing pDS7, and to Kirsten Sommer for providing pKS1. We would like to thank Barry Wanner for providing BW10724, Khoosheh Gosink and John S. Parkinson for providing pKG117 before publication, and Michael Manson and J.S. Parkinson for helpful discussions. This work was supported by a grant from the National Institute of General Medical Sciences (GM29481) to B.L. Taylor, and a Loma Linda University School of Medicine Research Seed Grant to M.S. Johnson.
The following supplementary material is available for this article online:
Table S1. Summary of Aer heterodimer results.
Table S2. Strains, plasmids and primers.
Fig. S1. Chemiluminescent Western blot showing the expression of mutant Aer proteins used for the complementation assays in Table 1. Expression was induced in BT3400 with 0.6 mM IPTG. WT Aer expression from pKW1 was approximately four to eightfold above chromosomal levels. Mw, molecular weight standards are shown.
Fig. S2. Determining the dominant or recessive character of Aer-HAMPM2 and Aer-proximal signallingM2 mutants with respect to Aer-WTM1 in BT3400. Aer mutants were coexpressed with WT Aer from compatible plasmids at approximately a 1:1 expression ratio. Dominant, but not recessive, Aer mutants impede WT Aer swarming on succinate semi-soft agar. Specifically, Aer-WTM1 was expressed from pDS7 and induced with either 0.5 mM or 1 mM sodium salicylate, while the Aer-HAMPM2 and Aer-proximal signallingM2 mutants were titrated against WT Aer with concentrations of IPTG between 0 and 0.6 mM. Western blots from cells in which the sodium salicylate/IPTG concentrations resulted in a 1:1 expression ratio are shown. To test for dominance, transformants were transferred to 30 mM succinate semi-soft agar containing these inducer concentrations and incubated at 30°C for 23 h.
A. Aer-L251PM2 was recessive to WT AerM1 at expression ratios ≥ 1:1.
B. Aer-C253RM2 was dominant over WT AerM1 at expression ratios ≤ 1:1.
C. Aer-L256RM2 was recessive to WT AerM1 at a 1:1 expression ratio.
D. Aer-D259HM2 was dominant over WT AerM1 at a 1:1 expression ratio.