The Spx paralogue MgsR (YqgZ) controls a subregulon within the general stress response of Bacillus subtilis
Article first published online: 14 JUL 2008
© 2008 Ernst-Moritz-Arndt-Universität Greifswald. Journal compilation © 2008 Blackwell Publishing Ltd
Volume 69, Issue 5, pages 1104–1120, September 2008
How to Cite
Reder, A., Höper, D., Weinberg, C., Gerth, U., Fraunholz, M. and Hecker, M. (2008), The Spx paralogue MgsR (YqgZ) controls a subregulon within the general stress response of Bacillus subtilis. Molecular Microbiology, 69: 1104–1120. doi: 10.1111/j.1365-2958.2008.06332.x
- Issue published online: 5 AUG 2008
- Article first published online: 14 JUL 2008
- Accepted 3 June, 2008.
The alternative sigma factor σB of Bacillus subtilis is responsible for the induction of the large general stress regulon comprising approximately 150–200 genes. YqgZ, a member of the σB regulon, resembles the global regulator Spx of the diamide stress regulon in B. subtilis. In this work we conducted a comprehensive transcriptome and proteome analysis of the B. subtilis wild-type 168 and its isogenic ΔsigB and ΔyqgZ mutants following exposure to 4% (v/v) ethanol stress, which led to the characterization of a ‘subregulon’ within the general stress response that is regulated by YqgZ. Activation and induction of σB are necessary but not sufficient for a full expression of all general stress genes. Expression of 53 genes was found to be positively regulated and the expression of 18 genes was negatively affected by YqgZ. The identification of the negatively regulated group represents a so far uncharacterized regulatory phenomenon observed in the ΔsigB mutant background that can now be attributed to the function of YqgZ. Due to the strict σB-dependent expression of YqgZ it was renamed to MgsR (modulator of the general stress response).