Present address: Omics Science Center, RIKEN Yokohama Institute, Kanagawa 230-0045, Japan.
Repression of small toxic protein synthesis by the Sib and OhsC small RNAs
Article first published online: 15 AUG 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd. No claim to original US government works
Volume 70, Issue 5, pages 1076–1093, December 2008
How to Cite
Fozo, E. M., Kawano, M., Fontaine, F., Kaya, Y., Mendieta, K. S., Jones, K. L., Ocampo, A., Rudd, K. E. and Storz, G. (2008), Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. Molecular Microbiology, 70: 1076–1093. doi: 10.1111/j.1365-2958.2008.06394.x
- Issue published online: 4 NOV 2008
- Article first published online: 15 AUG 2008
- Accepted 31 July, 2008.
The sequences encoding the QUAD1 RNAs were initially identified as four repeats in Escherichia coli. These repeats, herein renamed SIB, are conserved in closely related bacteria, although the number of repeats varies. All five Sib RNAs in E. coli MG1655 are expressed, and no phenotype was observed for a five-sib deletion strain. However, a phenotype reminiscent of plasmid addiction was observed for overexpression of the Sib RNAs, and further examination of the SIB repeat sequences revealed conserved open reading frames encoding highly hydrophobic 18- to 19-amino-acid proteins (Ibs) opposite each sib gene. The Ibs proteins were found to be toxic when overexpressed and this toxicity could be prevented by coexpression of the corresponding Sib RNA. Two other RNAs encoded divergently in the yfhL-acpS intergenic region were similarly found to encode a small hydrophobic protein (ShoB) and an antisense RNA regulator (OhsC). Overexpression of both IbsC and ShoB led to immediate changes in membrane potential suggesting both proteins affect the cell envelope. Whole genome expression analysis showed that overexpression of IbsC and ShoB, as well as the small hydrophobic LdrD and TisB proteins, has both overlapping and unique consequences for the cell.