Borrelia burgdorferi outer surface protein C (ospC) is required for the establishment of infection in mammals. However, its precise function remains controversial. The biologically active form of OspC appears to be a homodimer. Alpha helix 1 and 1′ of the apposing monomers form a solvent-accessible pocket at the dimeric interface that presents a putative ligand-binding domain (LBD1). Here we employ site-directed and allelic-exchange mutagenesis to test the hypothesis that LBD1 is a determinant of OspC function in the mammalian environment. Substitution of residues K60, E61 and E63 which line LBD1 resulted in the loss of infectivity or influenced dissemination. Analyses of the corresponding recombinant proteins demonstrated that the loss of function was not due to structural perturbation, impaired dimer formation or the loss of plasminogen binding. This study is the first to assess the involvement of individual residues and domains of OspC in its in vivo function. The data support the hypothesis that OspC interacts with a mammalian derived ligand that is critical for survival during early infection. These results shed new light on the structure–functions relationships of OspC and challenge existing hypotheses regarding OspC function in mammals.
This study focused on the functions of OspC associated specifically with the mammalian environment. Here, we employed site-directed mutagenesis to substitute conserved residues within LBD1 and used allelic exchange to replace the wild-type copy of ospC with single and double amino acid substitution mutants in an isogenic background. The mutant strains were then employed to test the hypothesis that the in vivo functional role of OspC in the mammalian environment is mediated by specific residues within LBD1. A significant distinction between this study and earlier analyses that have employed gene deletion/interruption approaches (Grimm et al., 2004; Pal et al., 2004; Tilly et al., 2006) is that we studied the role of individual residues and domains of OspC without the potential complication of perturbation of membrane organization and integrity. The results presented here suggest that an essential function of OspC is to bind a small mammalian-derived ligand via LBD1. These analyses represent a significant methodological and conceptual step forward in dissecting the sequence–structure–function relationships of one of the most intensively studied virulence factors of the Lyme disease spirochetes.
Site-directed mutagenesis of LBD1 of OspC of B. burgdorferi B31-5A4
To test the hypothesis that residues within LBD1 are involved in the in vivo functional activities of OspC that are required for the establishment of infection in mammals, residues K60, E61 and E63, which are charged, highly conserved, and possess side-chains that extend into the solvent accessible pocket of LBD1, were targeted for site-directed mutagenesis (Fig. 1E). These residues may serve as ligand contact points or be important in defining the physiochemical properties of LBD1 required for ligand binding. Residues E61 and E63, which are negatively charged and hydrophilic, and the positively charged and hydrophilic residue, K60, were replaced with Q and Y residues (polar and hydrophilic) respectively. We reasoned that these substitutions would alter charge distribution, a key parameter in ligand binding, without significantly affecting secondary structure. A master allelic-exchange construct (designated as pCAEV1) was generated and ospC genes harbouring amino acid substitutions were inserted. Selection was accomplished with a streptomycin resistance (strR) cassette downstream of the BbB21 open reading frame. In pCAEV1, the modified ospC genes are under the transcriptional control of a B. burgdorferi type A ospC promoter (Marconi et al., 1993a; Gilmore et al., 2001; Alverson et al., 2003; Yang et al., 2005; Xu et al., 2007). The endogenous cp26-encoded wild type-type A ospC gene of B. burgdorferi B31-5A4 was replaced with the mutated genes or with a pCAEV1 construct harbouring a wild-type copy of ospC; the latter of which served as a control to verify that the general genetic manipulation procedures employed did not alter the properties of B. burgdorferi B31-5A4 (detailed below). As a negative control, the cp26-carried wild-type copy of ospC was deleted from B. burgdorferi B31-5A4 and replaced with a resistance marker using the pΔospC strR vector (Fig. 2). After obtaining clonal populations of each mutant or control, plasmid content was determined through PCR using plasmid specific primer sets (data not shown) (Rogers et al., 2009). B31::ospC (wt), B31::ospC (K60Y) and B31::ospC (E61Q/E63Q) strains lost linear plasmid 21 (lp21) while the B31ΔospC, B31::ospC (E61Q) and B31::ospC (E63Q) strains retained the full complement of plasmids. As revealed by the infectivity data presented below, the loss of lp21 proved to be inconsequential. To verify that the allelic-exchange events did not result in unintended sequence changes, the introduced copy of ospC was amplified from the recombinant strains and the amplicons sequenced. No sequence alterations, other than those intended, were detected (data not shown).
Analysis of the expression, surface presentation and growth rates of strains expressing site-directed mutants of OspC
To verify that the site-directed mutants of OspC were produced, exported to the cell surface and distributed in the cell in a manner similar to that of wild-type OspC, immunoblot, proteinase K and immunofluorescence assays were performed. Immunoblot analyses demonstrated that OspC is produced at wild-type levels by each modified strain (Fig. 3A). Proteinase K digestion assays verified the export to and presentation of OspC in the outer membrane (Fig. 3A). The periplasmic flagellar protein, FlaB, was not cleaved by proteinase K, demonstrating that membrane integrity was maintained during proteolysis (Fig. 3A). This control served to further indicate that expression and presentation of the OspC site-directed mutants on the outer membrane of B31-5A4 did not, in and of itself, perturb membrane integrity and thus render cells more susceptible to proteolysis. Immunofluorescent assay (IFA) analyses demonstrated that the OspC proteins were uniformly distributed in the cell membrane. No aggregation or atypical clustering was observed (Fig. 3B). Lastly, growth curves revealed that the expression of the mutated OspC proteins does not modify growth rate (data not shown). Hence, all of the mutants displayed similar properties in vitro, indicating that there are no identifiable alterations that could potentially complicate comparative analyses of infectivity and dissemination of these strains in mice.
Analysis of the infectivity of isogenic strains expressing site-directed substitution mutants of OspC
To determine if the production of mutated OspC proteins affects the ability of each strain to infect and disseminate, recombinant strains were administered to mice using needle inoculation. Ear punch biopsies and urinary bladders were obtained 4 weeks after inoculation. Positive cultures were obtained from all mice infected with B31-5A4 (untransformed control), B31::ospC (wt), B31::ospC (E63Q) and B31::ospC (K60Y) (Table 1). In contrast, none of the mice injected with B31ΔospC, B31::ospC (E61Q/E63Q) or B31::ospC (E61Q) yielded positive cultures (Table 1). Since the sole genetic difference in strain B31::ospC (E61Q) relative to the wild-type parental strain was a single amino acid substitution, it can be concluded that E61 is a critical determinant of OspC function in mammals.
Table 1. Summary of culture results from mice inoculated with strains expressing wild-type and site-directed mutants of OspC.a
The data are presented as the number of mice determined to be infected based on positive cultures of the organ indicated after 2 or 4 weeks of incubation in media.
B31-5A4 (not transformed)
Analysis of the immune response to strains expressing site-directed mutants of OspC
To determine if the inoculated mice developed a significant IgG response to B. burgdorferi (i.e. seroconversion), anti-B. burgdorferi IgG titres were determined by ELISA with immobilized B31-5A4 whole cells serving as the test antigen. All strains that were infective, based on the cultivation results, displayed high-level IgG titres whereas strains that yielded negative cultivation results had low titres (Fig. 4A). The low titre antibody response observed in these strains most likely reflects a response to the inoculum (1 × 104 cells). Immunoblot analyses yielded results consistent with the ELISAs (Fig. 4B). Sera from all infected mice reacted strongly with multiple B. burgdorferi proteins while no significant reactivity of the sera derived from cultivation negative mice was observed. The ELISA and immunoblot results are consistent with the infectivity data and serve to further establish that specific site-directed substitutions within LBD1 result in a non-infectious phenotype.
Quantitative PCR analysis of the dissemination of infectious strains expressing site-directed OspC substitution mutants
To assess the influence of site-directed substitutions within LBD1 on dissemination, spirochete burdens in tissues and organs of all infected mice were determined by quantitative PCR (q-PCR). The parental B31-5A4 wild-type (untransformed), B31::ospC (wt) and B31::ospC (E63Q) strains disseminated to bladder, heart and joint (Fig. 5). The numbers of B31::ospC (E63Q) spirochetes in the bladders of infected mice were higher than those observed for all other strains; these differences were statistically significant. No significant differences in spirochete burdens were observed among the strains in tibiotarsal joints. Interestingly, very few B31::ospC (K60Y) spirochetes were detected in the heart. However, the difference was statistically significant only when compared with the wild-type strain and hence some caution is in order in assessing this data. Nonetheless, this observation is noteworthy in light of recent findings suggesting that OspC is a determinant required for colonization of the heart (Antonara et al., 2007).
Generation of recombinant OspC proteins harbouring site-directed mutations: determination of alpha helical content, ability to dimerize and electrostatic surface charge maps
The possible effect of each substitution on OspC secondary structure was assessed using multiple predictive algorithms. Little or no alteration of secondary structure was predicted (data not shown). To directly assess the effect of each substitution on alpha helical content, recombinant proteins were generated and circular dichroism spectroscopic analyses were conducted (Fig. 6). The estimated alpha helical content of the wild-type OspC protein (57.5 ± 4%) was found to be in excellent agreement with that determined by X-ray crystallography (∼58%) (Huang et al., 1999; Eicken et al., 2001; Kumaran et al., 2001). Estimated per cent alpha helical content values are indicated in Fig. 6. While the alpha helical content of E61Q decreased to some degree, the circular dichroism spectra of all other mutants were largely unaffected. To determine if the substitutions influenced the ability of OspC to dimerize, blue-native PAGE analyses were performed (Schagger et al., 1994) (Fig. 6, inset). All recombinant proteins efficiently dimerized and no residual monomer was detected. Collectively, these analyses indicate that the substitutions introduced into OspC did not significantly alter its structure or ability to dimerize.
To assess the possible impact of the amino acid substitutions on surface charge distribution, predictive surface charge maps were generated (Fig. 7). All substitution mutants displayed a surface charge map that differed in some domains from that observed for the wild-type protein. The maps for the E61Q and E63Q mutants were nearly indistinguishable with both mutants developing an expansive positive surface charge zone in and around LBD1 relative to the wild-type protein. The transition to a positively charged surface is even more evident in the E61Q/E63Q double mutant. This mutant also displayed a significant decrease in the distally located, negatively charged dome that is defined by LBD2. The K60Y protein also displayed significantly different surface charge distribution. Most of the positive charge clouds around the dimer were significantly reduced and transitioned to negative or neutral charge. The significance of the surface charge changes for each mutant is discussed below.
Plasminogen binding by recombinant OspC proteins
Plasminogen has been demonstrated to bind to recombinant OspC with the affinity of the interaction significantly influenced by OspC sequence (Lagal et al., 2006). The determinants of OspC required for plasminogen binding have not yet been identified. To determine if substitutions introduced into LBD1 affect plasminogen binding, ELISA analyses were conducted. With the exception of E61Q/E63Q, the binding levels showed only minor differences for the mutated proteins; interestingly, E61Q/E63Q displayed threefold greater binding relative to wild-type OspC (Fig. 8).
Using gene deletion approaches it has been demonstrated that OspC is required for the Lyme disease spirochetes to establish infection in mice using tick or needle routes of inoculation (Grimm et al., 2004; Pal et al., 2004; Tilly et al., 2006). While these analyses have advanced our understanding of when OspC participates in the enzootic cycle its biological function remains unknown. In this study, we tested the hypothesis that LBD1 is a key determinant of OspC function in the mammalian environment. Site-directed mutations were introduced into the type A ospC gene of B. burgdorferi and the mutated genes were transformed into an isogenic background. Residues K60, E61 and E63, which are invariant or highly conserved (Earnhart and Marconi, 2007) and which possess side-chains that extend into the solvent accessible pocket of LBD1, were targeted for mutagenesis (see Fig. 1). Analysis of the resulting recombinant strains revealed that the expression, export and presentation of the modified proteins on the cell surface were similar to the wild-type strain. All strains maintained wild-type growth rate and the full complement of plasmids (with the exception of the non-essential lp21 in some strains). Infectivity was assessed in mice through needle inoculation with cultivation and seroconversion serving as the read-out. The parental wild-type B31-5A4 strain (untransformed), B31::ospC (wt), B31::ospC (E63Q) and the B31::ospC (K60Y) strains were infectious while B31ΔospC, B31::ospC (E61Q/E63Q) and B31::ospC (E61Q) strains were non-infectious. It is striking that a single amino acid substitution at position 61 proved sufficient to render cells non-infectious, presumably by inactivating the critical functions associated with the OspC protein during the establishment of infection. For those strains that retained infectivity, we sought to determine if the amino acid substitutions introduced into LBD1 affect dissemination characteristics. The number of spirochetes present at different anatomical sites was determined by q-PCR. Higher numbers of B31::ospC (E63Q) in the urinary bladder and lower numbers of B31::ospC (K60Y) in the heart were observed. These results are consistent with the hypothesis that dissemination characteristics can be attributed, at least in part, to the physiochemical properties of OspC (Seinost et al., 1999; Brisson and Dykhuizen, 2004; Alghaferi et al., 2005; Earnhart et al., 2005; Jones et al., 2006; Wormser et al., 2008).
There are several possible explanations for the inability of the B31::ospC (E61Q) and B31::ospC (E61Q/E63Q) strains to establish infection. Since these strains are isogenic derivatives of the parental strain, the most direct explanation is that the loss of infectivity is a direct result of the modification of the OspC itself. The loss of infectivity would imply that OspC has a specific function during early-stage infection that cannot be fulfilled by any other Borrelia protein. As eluded to above, the biological properties of these and control strains suggest that they are not compromised in any way. At the molecular level, the amino acid substitutions could cause structural changes that ablate function. However, this possibility is not supported by secondary structure prediction analyses, circular dichroism and dimerization analyses that indicate that OspC structure and dimerization are largely unaffected. A second possibility is a direct role for residue E61 as a ligand contact point. Alternatively, E61 may indirectly influence the function of LBD1 through alteration of its local surface charge. While this possibility cannot be ruled out, it is noteworthy that the E63Q and E61Q mutants which are associated with infectivity and loss of infectivity, respectively, have nearly identical surface charge maps. In either case, it is clear that LBD1 and E61 are central in OspC's in vivo function.
The calculated size of the solvent accessible pocket of LBD1 suggests its potential ligands would be relatively small (Eicken et al., 2001; Kumaran et al., 2001). It was postulated in an earlier study that the region of OspC, which we refer to in this study as LBD1, may be an aspartate-binding domain (Eicken et al., 2001). The Salmonella typhimurium aspartate receptor, which is involved in chemotaxis and signalling (Yeh et al., 1996), possesses a four-alpha-helical bundle similar to that present in OspC. However, other than this limited structural similarity there is no discernible sequence homology between these proteins. While it seems unlikely that OspC binds aspartate, the general hypothesis that LBD1 is involved in the binding of small ligands is supported by its structural properties, the results presented herein and predictions obtained using the recently developed ConCavity algorithm (Capra et al., 2009). This algorithm, which combines information obtained from existing structural prediction algorithms, sequence conservation values and the 3D shape analysis, has been reported to perform well in identifying ligand binding pockets and functionally relevant residues (Capra et al., 2009). While the ConCavity program was not available at the time that the rationale for the site-directed mutagenesis analyses described here was devised, its predictions are in excellent agreement with the experimental results present within. ConCavity identified LBD1 as the highest probability ligand binding pocket of OspC with residues K60, A64, E61 and E63 having the highest predicted scores for involvement in ligand binding (see Fig. 1D). A64, which was not analysed in this report, appears to be part of a stabilizing, hydrophobic shell that lines the outer edge of the solvent accessible pocket of LBD1. While the identity of the ligands that interact with LBD1 remains unknown, there is now strong and compelling evidence that LBD1 is a key functional determinant of OspC that plays an essential role in the establishment of infection in mammals.
It is important to note that OspC has been reported to bind at least two other ligands, the Salp15 protein of Ixodes ticks (Das et al., 2001; Hovius et al., 2007) and the mammalian-derived protein plasminogen (Lagal et al., 2006). The domains and residues of OspC that are involved in these interaction have not been identified. In this study, we did not specifically assess Salp15–OspC interactions since our focus was to examine the functional role of OspC in the mammalian environment. Based on the properties of LBD1 and the fact that none of the LBD1 site-directed mutants displayed attenuated plasminogen binding, it appears that plasminogen interacts with regions outside of LBD1. Interestingly, the E61Q/E63Q protein bound plasminogen at a higher level than wild-type protein. Surface charge maps indicate that E61Q/E63Q has a significantly enhanced positive surface charge across the face of the dimer and a pronounced reduction in the negatively charged dome over LBD2. In that plasminogen binding to other proteins has been demonstrated to be mediated by electrostatic interactions (Rios-Steiner et al., 2001; Ye et al., 2001), it seems probable that the increased plasminogen binding by E61Q/E63Q reflects a more favourable surface charge on OspC. The results presented here highlight how sequence differences in natural OspC variants could affect plasminogen binding and possibly invasive potential. However, it is also evident that since the OspC mutant, E61Q, retains plasminogen binding ability but is non-infectious, it does not appear that plasminogen binding is the key function carried out by OspC. The extent to which the OspC–plasminogen interaction contributes to pathogenesis remains to be directly assessed. Such analyses are complicated by the fact that Borrelia produce several plasminogen-binding proteins including OspE and CspZ (factor H-binding proteins) (Hovis et al., 2006; Brissette et al., 2009).
Several opposing hypotheses have been offered regarding OspC function (reviewed in Radolf and Caimano, 2008). It has been postulated that OspC does not have a specific or unique function and instead, by simple virtue of its abundance, serves to maintain membrane integrity (Xu et al., 2008). It was reported that the introduction of random lipoprotein genes (OspE, OspA, VlsE and DbpA) on an autonomously replicating plasmid restored infectivity to an ospC knockout strain in SCID mice (but less so in immunocompetent mice). From this it was concluded that structurally unrelated proteins, none of which harbour a ligand binding pocket analogous to LBD1, are functionally equivalent (i.e. the ‘surrogate hypothesis’). If OspC can, in fact, be replaced by randomly selected, unrelated Borrelia lipoproteins, it is not clear why OspC variants with single amino acid substitutions that are unaltered in structure and expressed at wild-type levels cannot complement the putative membrane integrity maintenance function of OspC. In addition, if OspC could be replaced by other lipoproteins, it could be argued that ospC-deficient strains would have emerged in nature since there would be no selective advantage in maintaining such a gene. Extensive analyses of hundreds of Lyme disease isolates have clearly demonstrated that OspC is universal in all B. burgdorferi sensu lato complex species and homologues are carried by all relapsing fever spirochete species and isolates (Marconi et al., 1993b; Margolis et al., 1994). These facts, coupled with ospC phylogenetic analyses which indicate clear selective constraints on OspC sequence variation within specific domains, including LBD1 (Theisen et al., 1993; Baranton et al., 2001; Lagal et al., 2002; Lin et al., 2002; Attie et al., 2007; Earnhart and Marconi, 2007; Qiu et al., 2008), strongly argue against the ‘surrogate hypothesis’. Tilly et al. (2009) offered a slightly different interpretation on the ‘surrogate hypothesis’ in which it was proposed that OspC does have a specific function and that once OspC is downregulated in the mammalian environment, it is replaced by a surrogate protein that performs the same function. VlsE was suggested to be a possible OspC surrogate protein. However, as alluded to above, the divergent sequence and structure of VlsE and its lack of a LBD1-like domain suggest that VlsE is an unlikely surrogate for OspC.
This report represents a significant step forward as it is the first to provide direct evidence that a specific domain of OspC is required for in vivo function. Specifically, we have demonstrated that residue E61 is required for infectivity and provide suggestive evidence that residues E63 and K60 influence dissemination. While we do not conclude that these are the only functionally important residues of OspC, the data firmly support the importance of LBD1 in OspC function. The results presented here challenge recent hypotheses regarding the role of OspC in Borrelia pathogenesis. Consistent with the phylogenetic, structural and charge properties of OspC, we favour the hypothesis that OspC binds a small ligand that may be present in blood, extracellular fluid or possibly tissue that functions to facilitate the adaptive changes required for survival during the early stages of infection. Once the spirochetes adapt, OspC is no longer required and is downregulated. Such a model is consistent with the requirement of OspC for establishing infection in mice by tick or needle inoculation routes but not by tissue transplantation. In the latter case, the spirochetes would already be ‘host-adapted’ and would not require OspC. Although OspC lacks a transmembrane domain that might facilitate signalling, conformational changes in OspC, as a result of ligand binding, could trigger interactions with other cellular components that serve to transfer environmental signals and elicit adaptive responses. The approach employed here will allow for the further dissection of the essential determinants required for OspC function. With wild-type and ‘loss of function’ OspC mutant proteins in hand, it may be possible to devise high-throughput screening assays designed to identify OspC ligands that interact with LBD1 and thus unravel the function of OspC in Borrelia biology.
Site-directed mutagenesis of ospC
Site-directed mutations were introduced into the type A ospC gene derived from B. burgdorferi B31-5A4 by overlap extension using mutagenic primers (Table 2) as previously described (Hovis et al., 2008). Four mutants were generated: K60Y, E61Q, E63Q and E61Q/E63Q (a double mutant). The wild-type and mutant genes were annealed to the pET46 Ek/LIC vector and the plasmids were propagated as instructed by the supplier (Novagen). All constructs were validated by automated DNA sequencing (MWG Biotech).
Table 2. Oligonucleotides used in this study.
pCAEV1 upstream (+)
pCAEV1 vector construction
pCAEV1 OspC 5′ (−) BspEI/MluI
PCAEV1 vector construction
pCAEV1 OspC 3′ (+) BspEI
PCAEV1 vector construction
pCAEV1 BBB21 3′ (−) AatII
PCAEV1 vector construction
Spec/Strep (+) SalI
PCAEV1 vector construction
Spec/Strep (−) AatII
PCAEV1 vector construction
pCAEV1 downstream (+) SalI
PCAEV1 vector construction
pCAEV1 downstream (−) MluI
PCAEV1 vector construction
Insert type A (+) BspEI
Amplification of insert
Insert type A (−) BspEI
Amplification of insert
Upstream confirm (+)
Spec/strep 3′ (+)
Insert sequencing (+)
OspC sequence confirmation
Deletion upstream (+)
Deletion vector construction
Deletion upstream (−)
Deletion vector construction
Deletion downstream (+)
Deletion vector construction
Deletion downstream (−)
Deletion vector construction
OspC (+) LIC
Amplification of OspC for pET46
OspC (−) LIC
Amplification of OspC for pET46
OspC E61Q/E63Q (+)
OspC E61Q/E63Q (−)
OspC K60Y (+)
OspC K60Y (−)
OspC E61Q (+)
OspC E61Q (−)
OspC E63Q (+)
OspC E63Q (−)
FlaB q-PCR primer (+)
FlaB q-PCR primer (−)
Nid1 q-PCR primer (+)
Nid1 q-PCR primer (−)
Allelic-exchange replacement of wild-type ospC
A master allelic-exchange vector designated as pCAEV1 was generated using a pCR2.1 backbone and wild-type or mutated ospC genes were inserted (Fig. 2). To create pCAEV1 the following regions of B. burgdorferi B31-5A4 cp26 were amplified: (i) region 5′ of ospC (includes BbB18 and extends through the signal sequence and first four amino acids of OspC; the amplicon harbours 3′ tandem BspEI and MluI sites), (ii) region 3′ of ospC (includes BbB20 and BbB21 and flanking sequences; amplicon harbours 5′ BspEI and 3′ AatII sites), and (iii) region 3′ of BbB21 (includes a portion of BbB22; amplicon harbours 5′ SalI and 3′ MluI sites). To allow for streptomycin resistance selection, aadA was amplified from pKFSS1 using primers with 5′ SalI and 3′ AatII sites (Frank et al., 2003) (Table 2). All amplicons were individually annealed into the pCR2.1 TOPO plasmid and the plasmids were propagated in Escherichia coli. The plasmids were digested with the appropriate restriction enzymes to release the inserts. The inserts were ligated into the plasmid carrying the 5′ospC-containing fragment in the pCR2.1 TOPO vector and transformed into E. coli (C2925; New England Biolabs) (Fig. 1A). To create the ospC allelic-exchange vectors, wild-type or ospC genes with site-directed mutations (generated as detailed above) were amplified with primers possessing 5′ and 3′ BspEI sites. The amplicons were cut with the appropriate restriction enzyme and ligated into pCAEV1 (Fig. 2A). The plasmids were propagated in Novablue E. coli cells (Novagen) and purified (HiSpeed Plasmid Midi; Qiagen). The resulting plasmids were introduced into B. burgdorferi B31-5A4 (kindly provided by Dr Jon Skare) by electroporation essentially as previously described with some modifications (Samuels and Garon, 1993; Samuels et al., 1994). Cultures were grown at 33°C to mid-log phase in BSK-H medium with 6% rabbit serum (Sigma), collected by centrifugation, washed with cold Dulbecco's PBS and washed twice with cold EPS buffer (93 g l−1 sucrose, 15% glycerol). The cells (5 × 107) were collected and suspended in 150 µl of EPS buffer. Fifty microlitres of the cell suspension was mixed with 20 µg of plasmid linearized with MscI and ScaI-HF (New England Biolabs). After 5 min on ice, the cells were electroporated (0.2 cm cuvette, 2.5 kV, 25 µF, 200 Ω), transferred to 10 ml of BSK medium with 6% rabbit serum, and incubated overnight at 33°C. The culture volumes were increased to 50 ml with fresh BSK media, streptomycin (50 µg ml−1) was added, and the cultures were maintained at 33°C for 2–4 weeks. Clonal populations were obtained by subsurface plating. The plasmid contents of the transformants were determined by PCR using plasmid-specific primer sets (Mcdowell et al., 2001; Rogers et al., 2009). To determine the sequence of the DNA that crossed over into cp26, the desired region was PCR amplified and sequenced (MWG Biotech).
Generation of a B. burgdorferi B31ΔospC strain
A pΔospC strR knockout vector was created by amplification of the upstream (1000 bp) and downstream (1025 bp) regions of ospC with primers harbouring appropriate restriction sites. The upstream amplicon contains tandem AatII and AgeI sites at its 3′ end while the downstream amplicon has 5′ AatII and 3′ AgeI sites) (Table 2). The amplicons were cloned into pCR2.1 and the plasmids were propagated in Novablue E. coli. The plasmids were digested with AatII and AgeI, and the downstream fragment was ligated to the upstream-containing plasmid. Finally, the aadA gene (streptomycin/spectinomycin resistance) was excised from the pKFSS1-AatII plasmid (Frank et al., 2003) and ligated between the upstream and downstream sequences. Transformation, subsurface plating and plasmid screening were conducted as described above.
Assessment of growth curves
Fifteen millilitres of BSK-H complete medium was seeded with approximately 5 × 105 cells ml−1 actively growing cells. The culture was maintained at 33°C (5% CO2) and cell counts (in triplicate) were conducted each day using dark-field microscopy.
Production of recombinant OspC proteins and anti-OspC antiserum
For recombinant protein production, BL21 (DE3) cells were transformed with the pET46 Ek/LIC plasmids carrying wild-type or mutated ospC genes. Protein expression was induced with IPTG, the proteins purified by nickel chromatography, dialysed against three changes of PBS and quantified by the BCA assay (Pierce). To generate antiserum, C3H/HeJ mice were immunized with 10 µg of wild-type recombinant OspC protein adsorbed to Imject alum (Pierce), with boosts at weeks 4 and 6. Serum was collected by tail bleed.
Proteinase K proteolysis and immunofluorescence assays
To assess the presentation of OspC at the B. burgdorferi cell surface, strains were cultivated at 27°C and then transferred to 37°C for 3 days to upregulate ospC expression. The cells were harvested, washed twice with dPBS, and then the equivalent of 1 ml of culture at 0.3 OD600 was suspended in 1 ml of PBS with or without proteinase K (20 mg ml−1). The cell suspensions were incubated at 22°C (1 h), phenylmethylsulphonyl fluoride was added (0.5 mg ml−1 methanol), the cells were harvested and suspended in 120 µl of SDS-PAGE sample buffer. The cell lysates (2 µl) were subjected to SDS-PAGE, transferred to membranes and screened with mouse anti-OspC (1:20 000) or with mouse anti-FlaB (1:400 000). Antibody binding was detected using peroxidase-conjugated goat anti-mouse IgG and chemiluminescence (Supersignal West; Pierce).
The distribution of OspC on Borrelia cells was assessed by immunofluorescent microscopy. Cells were immobilized on slides (Superfrost Plus; Fisher), blocked with 3% BSA in PBS-T, and probed with mouse anti-OspC antiserum (1:2000). Alexafluor 488-conjugated rabbit anti-mouse IgG (1:200) served as the secondary antibody. The slides were mounted with Prolong Gold (Invitrogen) and the cells visualized using an Olympus BX51 fluorescence microscope.
Infectivity and dissemination analyses
Five C3H/HeJ mice per group were inoculated with 1 × 104 spirochetes of each strain by subcutaneous injection. After 4 weeks, the mice were euthanized and the heart, tibiotarsal joint, ear, bladder and blood were harvested. Portions of the ear and bladder biopsies were placed in BSK medium (supplemented with rifampicin, fosfomycin and amphotericin B) and incubated at 37°C under 5% CO2. Each sample was visually assessed for spirochetes by dark-field microscopy after 2 and 4 weeks of incubation. To isolate DNA, approximately 0.5 g of each tissue or organ was digested with collagenase (2 mg ml−1; 4 h; 37°C) and then with proteinase K (0.1 mg ml−1; 16 h; 55°C). The samples were extracted twice with phenol/chloroform/isoamyl alcohol, the DNA was precipitated using absolute ethanol, washed with 70% ethanol, suspended in water and quantified by UV spectrophotometery. For q-PCR, the Borrelia flaB gene and the mouse nidogen 1 (nid1) gene were amplified with Sybr Green PCR master mix (Applied Biosystems) using an Opticon 2 DNA engine (MJ Research). PCR cycling conditions were: 94°C for 10:00, 40 cycles of 94°C for 15 s, 56°C for 30 s, 72°C for 30 s, with fluorescence reads following the extension cycle. Relative numbers of gene copies were determined using a standard curve of Borrelia genomic DNA. To determine the extent of non-specific, background amplification, q-PCR was also conducted using DNA extracted from the organs and tissues of uninfected mice. All samples were run in three replicates of triplicates, and the organ-specific background amplification was subtracted from the mean ratios. Statistical significance was assessed by anova analysis of log-transformed data, with post hoc testing by the Holm-Sidak method (SigmaPlot).
ELISA and Western blotting
The humoral response to inoculation was assessed by whole-cell ELISA and Western blot analyses. For the ELISA analyses, B. burgdorferi B31-5A4 cells were collected, washed, suspended at 106 cells ml−1 (carbonate coating buffer) and immobilized onto ELISA plates (100 µl well−1; 4°C; overnight). The plates were blocked with 1% BSA in PBS-T. Serial dilutions of sera were applied (1 h) and antibody binding detected using peroxidase-conjugated goat anti-mouse IgG and ABTS chromogen. Titres were calculated as the inverse dilution corresponding to one-third of the plateau optical density. For Western blot analyses, B. burgdorferi B31-5A4 whole-cell lysates were separated by SDS-PAGE (Criterion, Bio-Rad) and blotted to PVDF membranes. The membranes were blocked with 5% non-fat dry milk in PBS-T and screened with mouse serum (1:1000 dilution). Antibody binding was detected using peroxidase-conjugated goat anti-mouse IgG and chemiluminescence (Supersignal West; Pierce).
Circular dichroism analysis of OspC
Far-UV circular dichroism spectra of wild-type and mutated recombinant OspC proteins were measured at 20°C in a Jasco J-715 spectropolarimeter (Jasco, Easton, MD, USA) (Chen et al., 1974). The protein samples (10 µM) were scanned in a 1 mm path-length cuvette using a 1 nm bandwidth, 8 s response time and a scan rate of 20 nm min−1. Three independent scans were made of each protein. Circular dichroism spectra were subtracted from background (buffer alone) and converted from circular dichroism intensity to molar ellipticity (deg cm2 dmol−1).
Analysis of OspC dimerization using blue-native PAGE
To determine if the recombinant wild-type and OspC site-directed mutant proteins undergo dimerization, 1 µg of each protein was equilibrated in blue-native PAGE loading buffer containing 0.02% Coomassie Brilliant Blue G-250 (CBB-G250), and electrophoresed in a Novex Bis/Tris NativePAGE gels 4–16% (Schagger et al., 1994). The cathode buffer (50 mM tricine, 15 mM bis/tris, pH 7.0) contained 0.002% CBB-G250 for the first half of the electrophoresis run. The anode buffer was 50 mM bis/tris pH 7.0. The molecular mass of the recombinant OspC proteins was interpolated from a standard curve generated with NativeMark (Invitrogen) marker mix.
Assessment of plasminogen binding by wild-type and site-directed mutant proteins
Plasminogen binding by recombinant OspC was assessed by ELISA (Lagal et al., 2006). ELISA plates (Costar 3590) were coated with 500 ng of plasminogen (P7999, Sigma) in carbonate buffer (pH 9.6). The wells were blocked with 1% BSA in PBS-T. Recombinant OspC proteins (1 µg well−1 in blocking buffer) were incubated in triplicate wells for 2 h at room temperature. Secondary detection of bound OspC was by mouse anti-His-tag monoclonal antibody (Pierce), then by peroxidase-conjugated goat anti-mouse IgG. Binding was quantified using ABTS chromogen.
We thank the members of the Marconi and Radolf labs for their helpful comments and suggestions. This work was supported in part by grants from the NIH, NIAID to R.T.M. (AI-67830) and J.D.R. (AI-29735).