†These authors contributed equally to this work.
YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB
Article first published online: 5 NOV 2010
© 2010 Blackwell Publishing Ltd
Volume 79, Issue 1, pages 109–118, January 2011
How to Cite
Tan, Q., Awano, N. and Inouye, M. (2011), YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB. Molecular Microbiology, 79: 109–118. doi: 10.1111/j.1365-2958.2010.07433.x
- Issue published online: 19 DEC 2010
- Article first published online: 5 NOV 2010
- Accepted manuscript online: 18 OCT 2010 12:00AM EST
- Accepted 7 October, 2010.
Toxin–antitoxin (TA) systems of free-living bacteria have recently demonstrated that these toxins inhibit cell growth by targeting essential functions of cellular metabolism. Here we show that YeeV toxin inhibits cell division, leads to a change in morphology and lysis of Escherichia coli cells. YeeV interacts with two essential cytoskeleton proteins, FtsZ and MreB. Purified YeeV inhibits both the GTPase activity and the GTP-dependent polymerization of FtsZ. YeeV also inhibits ATP-dependent polymerization of MreB. Truncated C-terminal deletions of YeeV result in elongation of cells, and a deletion of the first 15 amino acids from the N-terminus of YeeV caused lemon-shaped cell formation. The YeeV toxin is distinct from other well-studied toxins: it directs the binding of two cytoskeletal proteins and inhibits FtsZ and MreB simultaneously.