The authors declare no conflict of interest.
Absence of nucleoid occlusion effector Noc impairs formation of orthogonal FtsZ rings during Staphylococcus aureus cell division
Article first published online: 17 APR 2011
© 2011 Blackwell Publishing Ltd
Volume 80, Issue 5, pages 1366–1380, June 2011
How to Cite
Veiga, H., Jorge, A. M. and Pinho, M. G. (2011), Absence of nucleoid occlusion effector Noc impairs formation of orthogonal FtsZ rings during Staphylococcus aureus cell division. Molecular Microbiology, 80: 1366–1380. doi: 10.1111/j.1365-2958.2011.07651.x
- Issue published online: 25 MAY 2011
- Article first published online: 17 APR 2011
- Accepted manuscript online: 7 APR 2011 11:27PM EST
- Accepted 24 March, 2011.
The Gram-positive pathogen Staphylococcus aureus divides by synthesizing the septum in three orthogonal planes over three consecutive division cycles. This process has to be tightly coordinated with chromosome segregation to avoid bisection of the nucleoid by the septum. Here we show that deletion of the nucleoid occlusion effector Noc in S. aureus results in the formation of Z-rings over the nucleoid, as well as in DNA breaks, indicating that Noc has an important role as an antiguillotine checkpoint that prevents septa from forming over the DNA. Furthermore, Noc deleted cells show multiple Z-rings which are no longer placed in perpendicular planes. We propose that the axis of chromosome segregation has a role in determining the placement of the division septum. This is achieved via the action of Noc which restricts the placement of the division septum to one of an infinite number of potential division planes that exist in S. aureus.