Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix

Authors

  • S. M. Miller,

    1. Division of Gastroenterology and Hepatology, Department of Physiology & Biomedical Engineering, Mayo Clinic, Rochester, MN, USA
    Search for more papers by this author
  • R. A. Narasimhan,

    1. Enteric NeuroScience Program, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA
    Search for more papers by this author
    • 2

      Present address: Department of Medicine, Lahey Clinic, 41 Mall Road, Burlington, MA, USA.

  • P. F. Schmalz,

    1. Division of Gastroenterology and Hepatology, Department of Physiology & Biomedical Engineering, Mayo Clinic, Rochester, MN, USA
    Search for more papers by this author
  • E. E. Soffer,

    1. Department of Gastroenterology-Hepatology, Cleveland Clinic Foundation, Cleveland, OH, USA
    Search for more papers by this author
    • 1

      Present address: Division of Gastroenterology, Cedars-Sinai Hospital, Los Angeles, CA, USA.

  • R. M. Walsh,

    1. Department of Surgery, Cleveland Clinic Foundation, Cleveland, OH, USA
    Search for more papers by this author
  • V. Krishnamurthi,

    1. Department of Gastroenterology-Hepatology, Cleveland Clinic Foundation, Cleveland, OH, USA
    Search for more papers by this author
  • P. J. Pasricha,

    1. Division of Gastroenterology, University of Texas Medical Branch, Galveston, TX, USA
    Search for more papers by this author
  • J. H. Szurszewski,

    1. Division of Gastroenterology and Hepatology, Department of Physiology & Biomedical Engineering, Mayo Clinic, Rochester, MN, USA
    2. Enteric NeuroScience Program, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA
    Search for more papers by this author
  • G. Farrugia

    1. Division of Gastroenterology and Hepatology, Department of Physiology & Biomedical Engineering, Mayo Clinic, Rochester, MN, USA
    2. Enteric NeuroScience Program, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA
    Search for more papers by this author

Gianrico Farrugia MD, Division of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Tel: +1 507 284 4695; fax: +1 507 284 0266; e-mail: farrugia.gianrico@mayo.edu

Abstract

Abstract  The objective of this study was to determine the distribution of enteric nerves and interstitial cells of Cajal (ICC) in the normal human appendix and in type 1 diabetes. Appendixes were collected from patients with type 1 diabetes and from non-diabetic controls. Volumes of nerves and ICC were determined using 3-D reconstruction and neuronal nitric oxide synthase (nNOS) expressing neurons were counted. Enteric ganglia were found in the myenteric plexus region and within the longitudinal muscle. ICC were found throughout the muscle layers. In diabetes, c-Kit positive ICC volumes were significantly reduced as were nNOS expressing neurons. In conclusion, we describe the distribution of ICC and enteric nerves in health and in diabetes. The data also suggest that the human appendix, a readily available source of human tissue, may be useful model for the study of motility disorders.

Ancillary