Manometric features of eosinophilic esophagitis in esophageal pressure topography

Authors


Address for Correspondence
Sabine Roman, Division of Gastroenterology, Department of Medicine, Feinberg School of Medicine, Northwestern University, 676 St Clair St, Suite 1400, Chicago, IL 60611-2951, USA.
Tel: +312 695 4016; fax: +312 695 3999;
e-mail: roman.sabine@gmail.com

Abstract

Background  Although most of the patients with eosinophilic esophagitis (EoE) have mucosal and structural changes that could potentially explain their symptoms, it is unclear whether EoE is associated with abnormal esophageal motor function. The aims of this study were to evaluate the esophageal pressure topography (EPT) findings in EoE and to compare them with controls and patients with gastro-esophageal disease (GERD).

Methods  Esophageal pressure topography studies in 48 EoE patients, 48 GERD patients, and 50 controls were compared. The esophageal contractile pattern was described for ten 5-mL swallows for each subject and each swallow was secondarily characterized based on the bolus pressurization pattern: absent, pan-esophageal pressurization, or compartmentalized distal pressurization.

Key Results  Thirty-seven percent of EoE patients were classified as having abnormal esophageal motility. The most frequent diagnoses were of weak peristalsis and frequent failed peristalsis. Although motility disorders were more frequent in EoE patients than in controls, the prevalence and type were similar to those observed in GERD patients (P = 0.61, chi-square test). Pan-esophageal pressurization was present in 17% of EoE and 2% of GERD patients while compartmentalized pressurization was present in 19% of EoE and 10% of GERD patients. These patterns were not seen in control subjects.

Conclusions & Inferences  The prevalence of abnormal esophageal motility in EoE was approximately 37% and was similar in frequency and type to motor patterns observed in GERD. Eosinophilic esophagitis patients were more likely to have abnormal bolus pressurization patterns during swallowing and we hypothesize that this may be a manifestation of reduced esophageal compliance.

Abbreviations:
CFV

contractile front velocity

DCI

distal contractile integral

EGJ

esophagogastric junction

EoE

eosinophilic esophagitis

EPT

esophageal pressure topography

FLIP

functional luminal imaging probe

GERD

gastro-esophageal reflux disease

HRM

high resolution manometry

IBP

intra bolus pressure

IRP

integrative relaxation pressure

LES

lower esophageal sphincter

PPI

proton pump inhibitors

Introduction

Intermittent dysphagia and food impaction are the most common symptoms in adult patients with eosinophilic esophagitis (EoE).1 The typical findings on endoscopy include mucosal rings, linear furrows, and white papules. In some patients, the endoscopic pattern may explain the cause for dysphagia, especially in the cases of esophageal strictures or small caliber esophagus.2 However, mucosal rings are often widely patent in EoE patients or dysphagia may occur without any significant endoscopic findings.

Esophageal dysmotility has been proposed as an alternative explanation for dysphagia in EoE patients outside of the overt mechanical obstruction associated with rings and stenosis. Conventional manometric studies assessing esophageal motor function in EoE have reported varied results ranging from normal peristalsis to absent peristalsis as well as motility disorders including achalasia, diffuse esophageal spasm, nutcracker esophagus, high amplitude contractions, ineffective peristalsis, and nonspecific motor disorders. In a previous review using conventional manometry, Nurko et al. noted that abnormal motility findings may be seen in 41% of patients3 and that nonspecific disorders were the most frequent abnormalities encountered (71% of patients with abnormal manometric findings).

Although the histopathology of EoE is primarily defined by the infiltration of the mucosa with eosinophils, there are other features that may promote esophageal dysmotility. Increased contraction of fibroblasts has been observed with eosinophils and fibroblasts in co-culture4 and axonal necrosis has been described in EoE.5 Additionally, the control of esophageal contraction could potentially be modified as the binding between eosinophil major protein and muscarinic acetylcholine receptors will promote smooth muscle contraction.6 Finally, eosinophils have profibrogenic properties and chronic inflammation with eosinophils may induce a secondary fibrosis.7 Although fibrosis may be responsible for the stiffness of the esophageal wall encountered in many EoE patients, it could also potentially disrupt esophageal contractile function.

High resolution manometry (HRM) displayed as esophageal pressure topography (EPT) is becoming a widely accepted technique for evaluating and categorizing esophageal motility disorders in clinical practice because of its technical advantages over conventional manometry.8 However, HRM and EPT findings in EoE have not yet been reported other than in small case series or case reports.9,10 Our preliminary findings using EPT suggest that EoE patients exhibit abnormalities in pressurization patterns consistent with reduced esophageal compliance and functional outflow obstruction. Thus, the aim of this study was to evaluate both the contractile and pressurization patterns observed in EPT studies of EoE patients and to compare the results with controls and subjects with gastro-esophageal reflux disease (GERD).

Patients and methods

Subjects

We identified 48 patients diagnosed with EoE who had active disease at the time of manometry from a consecutive series of 2 500 clinical high resolution EPT studies conducted between August 2004 and May 2010. The diagnosis of EoE was based on esophageal symptoms suggestive of EoE and endoscopic biopsies with histopathology confirming EoE (>15 eos/hpf; magnification 0.196 mm2).11 Reflux monitoring was not consistently performed to exclude GERD in these patients. From the same series, we identified 48 consecutive patients with GERD verified by symptom profile and ambulatory reflux testing. Gastro-esophageal reflux disease patients were defined by either esophageal acid exposure >5% (n = 44) while not taking a proton pump inhibitors (PPI)12,13 or greater than 73 reflux events in 24 h detected on impedance monitoring (n = 4).14 Subjects with previous upper gastrointestinal (GI) surgery were excluded from the study. These groups were compared to 50 healthy volunteers randomly selected from a database of 75 healthy volunteers.

The study protocol for healthy volunteers was approved by the Northwestern University Institutional Review Board (PJK) and informed consent was obtained from each subject. The study protocol for the retrospective analysis of EPT findings in the EoE and GERD patients was also approved by the Northwestern University Institutional Review Board (JEP).

EPT protocol

Esophageal pressure topography studies were done with a 4.2 mm outer diameter solid-state assembly with 36 circumferential sensors spaced at 1-cm intervals (Sierra Scientific Instruments, Los Angeles, CA, USA). Before recording, transducers were calibrated at 0 and 300 mmHg using externally applied pressure. Studies were done in a supine position after at least a 6-h fasting period. The manometry assembly was placed transnasally and positioned to record from the hypopharynx to the stomach with about three intra-gastric sensors. The catheter was fixed in place by taping it to the nose. The manometric protocol included at least a 30-s period to assess basal sphincter pressure and ten 5-mL water swallows. In some subjects two 10-mL and one 20-mL water swallows were performed.

EPT Analysis

Esophageal pressure topography data were analyzed using manoview™ analysis software (Sierra Scientific Instruments). Esophagogastric junction (EGJ) morphology was classified as type I when separation between the highest pressure of the lower esophageal sphincter (LES) and crural diaphragm was indiscernible (<1 cm), type II when LES-crural diaphragm separation was >1 cm and <2 cm and type III when LES-crural diaphragm separation was >2 cm. End-expiratory and end-inspiratory EGJ pressure, and intragastric pressure were measured during the basal period without swallowing. Esophagogastric junction relaxation pressure was assessed with the 4-s integrated relaxation pressure (4-s IRP) for the ten 5-mL swallows.15

Each swallow was further characterized using the isobaric contour tool to determine the location and length of breaks in peristalsis if present. The distal contractile integral (DCI), the contractile front velocity (CFV) between the transition zone and the contractile deceleration point, and the maximal intra bolus pressure (IBP) were also measured for each swallow.16,17 The individual swallow contractile patterns were categorized using the above parameters into five patterns as specified in Table 1.

Table 1.   Contraction patterns using EPT
Contraction patternEsophageal pressure topography characteristics
  1. Modified from Pandolfino et al.16

  2. CFV, contractile front velocity; DCI, distal contractile integral.

FailedMinimal (<3 cm) integrity of the 20 mmHg isobaric contour distal to the proximal pressure through
Weak peristalsisPresence of a break in the 20 mmHg isobaric contour greater than 2 cm
Subdivided into small (2–5 cm) and large (>5 cm) breaks
HypertensiveNormal CFV with DCI >5 000 mmHg cm s
Rapidly propagatedCFV ≥ 10 cm s−1
NormalNot achieving any of the above diagnostic criteria

Elevated IBP was defined as exceeding 25 mm-Hg referenced to atmospheric pressure. This threshold was chosen based on data assessing flow permissive pressures through the EGJ and confirmed by assessing the 95th percentile range of IBP in asymptomatic control subjects.17,18 The presence of elevated IBP was further characterized as: (i) pan-esophageal pressurization when it spanned the length of the esophagus, or (ii) compartmentalized distal esophageal pressurization when the pressurization extended distally from the contractile front to the EGJ (Fig. 1). Moreover, IBP was measured for each swallow 2 cm above the proximal border of the EGJ and defined as the greatest pressure for a 3-s period (contiguous or non-contiguous) within the same temporal boundaries used to calculate the IRP.19 The resultant maximal IBP for each subject was calculated as the mean of the three greatest single swallow values as this was previously established as optimal discriminator.19

Figure 1.

 Examples of different pressurization patterns at the 25-mmHg isobaric contour in patients with eosinophilic esophagitis. The early pan-esophageal pressurization occurred in the context of normal peristalsis and normal IRP. The compartmentalized distal pressurization occurred in a patient with normal peristalsis and normal mean IRP (9.5 mmHg). For this particular swallow, the IRP is slightly elevated (16.6 mmHg).

Based on the different metrics and the pattern of the contractile activity for the ten 5-mL swallows, esophageal motility disorders were diagnosed and categorized using the most recent published version of the Chicago classification16 modified for the diagnosis of weak peristalsis (>20% swallows with large breaks or >30% of small breaks) and frequent failed peristalsis (40–90% of failed swallows).20

Statistical analysis

Data were expressed as median (interquartile range) unless otherwise specified. Among the three groups, categorical data were compared using chi-square test and continuous data using Mann–Whitney or Kruskal–Wallis tests. A P-value less than 0.05 was considered as significant.

Results

Demographic, clinical and endoscopic characteristics of the subjects

The demographic and clinical characteristics of the subjects are summarized in Table 2. The control subjects were significantly younger than the EoE and GERD patients (P < 0.01) and the EoE patients were also younger than the GERD patients (P = 0.049). All of the EoE patients but two reported having dysphagia at the time of the HRM study.

Table 2.   Demographic, clinical, and endoscopic characteristics of the subject groups
 Control subjectsEoE patientsGERD patients
  1. NA, not available.

Number504848
Mean age (range) years28 (19–48)42 (19–77)48 (20–83)
Gender (male/female)29/2128/2023/25
Symptoms
 Dysphagia046 (96%)4 (8%)
 Food impaction028 (58%)0
 Heartburn ±   regurgitation022 (46%)36 (75%)
 Atypical GERD  symptoms01 (2%)12 (25%)
Endoscopy
 RingsNA26 (54%)0
 FurrowsNA29 (60%)0
 StrictureNA7 (15%)0
 Schatzki ringNA9 (19%)2 (4%)
 Hiatus herniaNA1 (2%)12 (25%)
 Esophagitis (Los Angeles classification)NA07 (15%) grade A,
 3 (6%) grade B,
 1 (2%) grade D

Endoscopic examination revealed esophageal rings associated with furrows in 18 EoE patients; seven were judged to have a dominant ring. Ten EoE patients had no rings or furrows: two had mucosal exudates, two had a distal Schatzki ring, two had a distal stricture, one had narrowing of the distal esophagus and four had a normal endoscopy. One GERD patient had esophagitis dissecans superficialis; in this case the biopsies were negative for eosinophils. Additionally, 20 of the GERD patients had esophageal biopsies that were negative for eosinophils, while 27 did not have biopsies for evaluation.

EPT in the three subject groups

The majority of subjects had type I EGJ morphology. One control, one EoE and four GERD patients had type II EGJ morphology and two EoE and nine GERD patients had type III EGJ morphology. As shown in Table 3, baseline EGJ pressure was statistically similar among the three groups; however, the GERD patients had a numerical trend toward lower end-expiratory EGJ pressure. The mean IRP was normal in all but one control (IRP = 16 mmHg) and elevated in five EoE patients (17–23 mmHg) and three GERD patients (15–22).

Table 3.   Manometric data in the different groups of subjects. Data are expressed as median (interquartile range) unless otherwise specified
 ControlsEoE patientsGERD patientsP (Kruskal–Wallis test)
  1. EGJ, esophagogastric junction; IRP, integrated relaxation pressure.

  2. *P < 0.05 vs healthy volunteers (Mann–Whitney test).

  3. P < 0.05 vs GERD patients (Mann–Whitney test).

  4. **Chi-square test.

  5. P < 0.05 vs healthy volunteers (chi-square test).

  6. P < 0.05 vs GERD patients (chi-square test).

Expiratory EGJ pressure (mmHg)15 (9–22)18 (8–31)10 (5–18)*0.04
Inspiratory EGJ pressure (mmHg)21 (16–28)23 (14–40)20 (11–28)0.24
IRP (mmHg)8.4 (6.1–10)7.8 (6.2–10.9)7.3 (4.4–9.4)0.17
Normal peristalsis (n)10 (9–10)8 (3–10)*7 (2–9)*<0.01
Failed peristalsis (n)0 (0–0)0 (0–2)*0 (0–1)*<0.01
Weak peristalsis (n)0 (0–1)1 (0–2)*2 (1–6)*<0.01
Pressurization
 Pan-esophageal, n (%)0 (0%)8 (17%)¶‡1 (2%)<0.01**
 Compartmentalized distal, n (%)0 (0%)9 (19%)¶‡5 (10%)

Figure 2 depicts the distribution of EPT diagnoses among the controls, EoE, and GERD patients. Motility disorders were significantly more frequent in EoE and GERD patients than in controls (P = 0.02) but the occurrence and the type of motility disorders did not differ between GERD patients and EoE patients (P = 0.61). Weak peristalsis and frequent failed peristalsis were the most common diagnoses observed in EoE and GERD patients. This was consistent with the observation that EoE and GERD patients had significantly more swallows with failed and weak peristalsis than controls (Table 3). The subgroup of GERD patients with esophageal biopsies negative for eosinophils was also not different from the EoE patients (P = 0.25).

Figure 2.

 Distribution of manometric diagnosis in controls, EoE, and GERD patients. Weak peristalsis, frequent failed peristalsis, hypertensive peristalsis and rapid contraction with normal latency are present in the three groups of subjects in different proportions. The motility disorders were more frequent in EoE and GERD patients (P = 0.02) but no significant difference was observed between EoE and GERD patients (P = 0.61) The distribution of manometric diagnosis is also shown in the subgroup of GERD patients with negative esophageal biopsies and was not significantly different from that of the EoE patients (P = 0.25).

Esophageal pressurization

The distribution of pressurization patterns among subject groups is shown in Table 3. There was a significant difference in the prevalence of abnormal pressurization (P < 0.01) among the three groups. It is important to note that abnormal pressurization was not observed in any control subject. However, abnormal pressurization patterns were found in 36% of EoE patients (pan-esophageal pressurization 17%; compartmentalized distal pressurization 19%) and 12% of GERD patients (pan-esophageal pressurization 2%; compartmentalized distal pressurization 10%). The pan-esophageal pressurization occurred within 2-s of the esophageal contraction (early pan-esophageal pressurization) in all but one EoE patient. When pressurization was present the median (range) number of affected swallows per subject was one (1–9) for pan-esophageal pressurization and two (1–9) for compartmentalized distal pressurization in EoE patients. In the group of GERD patients, the only subject with pan-esophageal pressurization had only one swallow affected and the median (range) number of swallows with compartmentalized pressurization was similar to what was observed in EoE patients [2(1–8), P = 0.45, Mann–Whitney test]. Figure 3 illustrates the distribution of maximal IBP among the three subject groups. There was no difference between controls and EoE patients (P = 0.1) whereas GERD patients had a lower IBP than controls (P = 0.02) or EoE patients (P < 0.01).

Figure 3.

 Distribution of the maximal IBP among the controls, EoE and GERD patients. The distribution was significantly different between controls and GERD (P = 0.02) and between EoE and GERD (P = 0.01), but not between controls and EoE (P = 0.1).

The majority of EoE patients with abnormal pressurization had normal EGJ findings (76%) with only three patients exhibiting an elevated IRP and one subject exhibiting type III EGJ morphology. Of the 17 patients with abnormal pressurization; two had a normal endoscopy while the other patients had endoscopic features consistent with EoE. Seven of the EoE patients with abnormal pressurization had obstructive findings on endoscopy (Schatzki ring, stricture, esophageal narrowing, or dominant stricture) while ten did not (P = 1.00). All but one of the GERD patients with abnormal pressurization had esophagitis; one subject had a normal endoscopy with an elevated IRP. Only two of the 21 GERD patients with biopsies negative for eosinophils had an instance of esophageal pressurization (one pan-esophageal pressurization and one compartmentalized distal pressurization).

Pressurization for 5-, 10- and 20-mL swallows

Ten-mL test swallows were performed in 32 controls, 38 EoE and 23 GERD patients; 20-mL swallows in 32 controls, 37 EoE and 12 GERD patients. Comparing only for the final 5- and 10-mL swallow to the 20 mL swallows, the bolus challenge increased the number of swallows with pressurization in the control subjects and EoE patients. Thirty-two percent of EoE patients had early pan-esophageal pressurization for the 20-mL swallow vs 3% of controls and 8% of GERD patients (P = 0.04) (Fig. 4).

Figure 4.

 Percentage of subjects with pressurization at 25-mmHg isobaric contour for 5, 10, and 20-mL swallows. The number of subjects with pressurization was significantly different among the three groups (P = 0.04).

Discussion

This study represents the first systematic investigation of EPT findings in EoE patients, comparing them to normal control subjects and GERD patients. Abnormal EPT was encountered in 37% of EoE patients with the most frequent diagnoses being weak peristalsis and frequent failed peristalsis. While these findings distinguish the EoE group from the control subjects, the same spectrum of diagnoses were encountered in GERD patients. However, EoE patients were much more likely to have abnormal bolus pressurization in the esophagus compared to GERD patients or control subjects and it appeared that early pan-esophageal pressurization after swallowing was a specific finding in EoE.

Various esophageal motility disorders have been described in previous smaller series of adult and pediatric EoE patients using conventional manometry.10,21–25 These studies report a wide range of prevalence rates for esophageal motor abnormalities (22–90%) with the most frequent observed disorders being weak and failed peristalsis. However, no control populations were included in these studies and the motility patterns encountered do not seem to be specific for EoE. In fact, our data suggest that although the frequency of weak and failed peristalsis is increased in EoE, this finding is similar to what one observes in a GERD population. This finding is intriguing given the suggested clinical overlap between GERD and EoE.

A novel finding in this study was that increased bolus pressurization occurred in significantly more EoE patients than GERD patients or control subjects. A unique feature of EPT is the ability to assess pressurization patterns in the esophagus that occur independent of lumen-obliterating contractions. More than one-third of the EoE patients had abnormalities in pressurization noted on EPT, compared to only 12% of GERD patients and none of the control subjects. Although these findings suggest some overlap with GERD, most of the overlap was associated with the pattern of distal compartmentalized pressurization in the context of abnormal EGJ structure or inflammation. Maximal IBP appears to be less useful than the panesophageal pressurization pattern to discriminate EoE patients from controls. This may be partly a methodological consideration as we measured the maximal IBP sustained for a period of 3 s and pan-esophageal pressurization is generally sustained for only shorter periods (Fig. 1). The pattern of early pan-esophageal pressurization was unique to EoE patients during 5 mL swallows and this pattern of pressurization was more easily elicited during large volume (20 mL) swallows. We hypothesize that this pressurization pattern is a manifestation of reduced compliance in the esophagus. Recent work utilizing the functional luminal imaging probe (FLIP), a modification of impedance planimetry, to assess the luminal cross-sectional area during pressure distention, reported reduced esophageal wall distensibility in EoE compared to healthy control subjects.26 An alternative explanation for the pattern of early pan-esophageal pressurization would be exaggerated longitudinal muscle contraction and esophageal compression. That explanation, however, is unlikely as there is no evidence of augmented esophageal shortening in this patient population. Recent work utilizing intraluminal ultrasound suggests that longitudinal muscle contraction is reduced in EoE and further blunted after pharmacologic stimulation.27

Our study has some limitations attributable to its retrospective design. Our control subjects were younger than both the EoE and the GERD patient groups and, thus, we cannot rule out an age effect on the occurrence of weak and failed peristalsis. However the distribution of age, although statistically different, was similar among EoE and GERD patients (42 vs 48) and the pattern of early pan-esophageal pressurization was much more frequent in EoE patients than in GERD (and completely absent in control subjects). We also have no data regarding esophageal acid exposure in our EoE patients and it is possible that some of the overlap in EPT findings between GERD and EoE could be secondary to reflux. However, this would not alter the observation that EoE is not associated with unique EPT diagnoses as the prevalence of weak and frequent failed peristalsis would be reduced if one excluded the potential effect of abnormal acid exposure and the associated peristaltic abnormalities that co-exist with GERD. Vice-versa, there is also a possibility that some of the GERD patients could have overlap with EoE based on the fact that we only had histopathologic data on 21 GERD patients. However, our subanalysis utilizing only GERD patients with a negative biopsy did not show a significant difference in the prevalence of contractile patterns between EoE and GERD patients with negative histopathology.

In conclusion, this study suggests that the most common EPT abnormalities found in EoE, weak peristalsis and frequent failed peristalsis, occur with similar frequency in EoE and GERD. Hence, neither is specific for EoE. However, pan-esophageal pressurization after swallowing in conjunction with normal EGJ relaxation was an EPT finding almost exclusive to EoE. This pattern of pressurization can be accentuated with larger volume swallows and is likely related to decreased esophageal compliance in EoE. Whether this finding is associated with more advanced disease or whether it can be utilized to monitor disease activity will require pre- and post-intervention studies with validated symptom end-points.

Acknowledgments

This work was supported by NIH grants RO1 DK056033 (PJK) and RO1 DK079902 (JEP) from the Public Health Service.

Author contribution

SR was involved in analysis and interpretation of data, and drafting of the manuscript; IH was involved in study concept and design, analysis and interpretation of data; MK, NG and JC were involved in analysis and interpretation of data; PJK and JEP were involved in study concept and design, analysis and interpretation of data, and drafting of the manuscript.

Competing interest

The authors have no competing interests.

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