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Figure S1. The effect of the EP1 receptor antagonist, EP1A (histograms to the left); the EP3 antagonist, L798106 (middle) or the EP4 receptor antagonist, GW627368X (right) on PGE2 responses in human colon (a), mouse colon (b), and mouse ileum (c). Each bar represents the mean ± 1 SE mean % change in basal tension, normalized to the maximal contraction induced by CCh (10 μmol L−1), and the n numbers are shown in parentheses. Significant differences between control PGE2 ± an EP receptor antagonist are as follows: *P < 0.05, **P < 0.01, ***P < 0.001.

Figure S2. Pooled responses to veratridine and veratridine after 15 min pretreatment with lidocaine in mouse (a) colon and (b) ileum. Note that veratridine decreased longitudinal smooth muscle tension of mouse colon, whereas the depolarizing agent induced a biphasic response with a predominant increase in tension in the ileum; and the separate phases in the latter are pooled as separate bars. Lidocaine abolished veratridine responses, without any effect on subsequent CCh (10 μmol L−1)-induced contractions, in mouse (c) colon and (d) ileum. In (a) and (b), each bar represents the mean ± 1 SE mean % change in basal tension, normalized to the maximal contraction induced by CCh (10 μmol L−1). In (c) and (d), each bar is the mean ± 1 SE mean absolute change in basal tension. The n numbers throughout are shown in parentheses. Significant differences between control veratridine and veratridine after lidocaine are represented as follows: *P < 0.05, ***P < 0.001.

Figure S3. Representative responses to capsaicin (Caps) in mouse (a) colon and (b) ileum. The first addition of capsaicin induced a sustained decrease in tension, which was re-adjusted manually back to 1 g after 10 min. A subsequent addition of capsaicin, 5 min later, did not induce a response in either tissue.

Figure S4. Single additions of CCh, nicotine (Nic) and substance P (SP) in the absence or presence of the following combined antagonist pretreatment, hexamethonium (1 μmol L−1); atropine (10 μmol L−1); RP67580 (2 μmol L−1) and MEN10627 (1 μmol L−1; +antagonists) in mouse colon. Each bar shows the mean ± 1 SE mean absolute change in basal tension and the n numbers are shown in parentheses. Significant differences between CCh and SP responses in the absence or presence of the combined antagonist pretreatment are as follows: *P < 0.05, ***P < 0.001.

Figure S5. VIP decreased longitudinal smooth muscle tension of mouse ileum and these responses were inhibited in a concentration-dependent manner by the VPACI receptor antagonist, PG97269. Each bar shows the mean ± 1 SE mean % change in basal tension, normalized to the maximal response induced by CCh (10 μmol L−1), and the n numbers are shown in parentheses. Significant differences between control VIP and VIP following PG97269 pretreatment are represented as follows: *P < 0.05, **P < 0.01.

Table S1. Comparison of PGE2 (300 nmol L−1) and CCh (10 μmol L−1) responses in longitudinal smooth muscle preparations from male and female patients and from either descending colon or ascending colon specimens. Descending colon was obtained after anterior resected tissue and ascending colon from hemi-colectomies. Values are the mean ± 1 SE mean (with n numbers) and there were no significant differences between data groups with either agonist.

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NMO_1727_sm_FigS3.tif78KSupporting info item
NMO_1727_sm_FigS4.tif7KSupporting info item
NMO_1727_sm_FigS5.tif6KSupporting info item
NMO_1727_sm_TableS1.doc32KSupporting info item

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