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Figure S1. Detection of neuronal nitric oxide synthase (Nos1) dimers by Western immunoblotting.

Figure S2. Flow cytometry analysis of interstitial cells of Cajal (ICC) and their precursors.

Figure S3. Detection of Klotho expression in the small and large intestines of WT and klotho mice.

Figure S4. Quantification of ICC and ICC-SC by flow cytometry (representative projections).

Table S1. Antibodies used in Western immunoblotting studies.

Table S2. Antibodies used in immunohistochemistry studies.

Table S3. Antibodies used for flow cytometry of cells freshly dissociated from small intestinal and colonic muscles.

Table S4. Configuration of the Becton Dickinson LSR II flow cytometer.

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