Rosiglitazone reverses increased duodenal inhibitory response in spontaneously hypertensive rats
Article first published online: 14 OCT 2011
© 2011 Blackwell Publishing Ltd
Neurogastroenterology & Motility
Volume 24, Issue 1, pages e56–e66, January 2012
How to Cite
De Benedictis, L., Potenza, M. A., Gagliardi, S., Zigrino, A., Montagnani, M. and De Salvia, M. A. (2012), Rosiglitazone reverses increased duodenal inhibitory response in spontaneously hypertensive rats. Neurogastroenterology & Motility, 24: e56–e66. doi: 10.1111/j.1365-2982.2011.01798.x
- Issue published online: 21 DEC 2011
- Article first published online: 14 OCT 2011
- Received: 18 April 2011 Accepted for publication: 16 September 2011
Figure S1. Green and red immunofluorescence negative controls.
Figure S2. Expression of HO-1 and possible colocalization with PGP9.5 and DAPI in duodenum from WKY, vehicle-treated SHR, and ROSI- treated SHR rats. Representatives photomicrographs of duodenum sections immunostained with antibodies against HO-1 (C, G, K), PGP9.5 (D, H, L), DAPI (E, I, M), and superimposed images (F, J, N). Visualization by inverted microscopy shows: (first row) immunoreactivity red fluorescence-associated staining for HO-1 in nerve cell bodies of myenteric plexus between the circular (cm) and the longitudinal muscle (lm) layers in duodenum sections from WKY (C), vehicle-treated SHR (G), and ROSI-treated SHR rats (K); second row shows green fluorescence-associated staining for PGP9.5, neuronal marker, in duodenum section from WKY (D), vehicle-treated SHR (H), and ROSI-treated SHR rats (L); third row shows blue fluorescence-associated staining for DAPI, nuclear marker, in duodenum section from WKY (E), vehicle-treated SHR (I), and ROSI-treated SHR rats (M); fourth row shows merge of green, red, and blue fluorescence indicative of co-localization for HO-1, PGP9.5 (yellow fluorescence), and DAPI in WKY (F), vehicle-treated SHR (J), and ROSI-treated SHR rats (N). ×630 magnification.
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