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Figure S1. Green and red immunofluorescence negative controls.

Figure S2. Expression of HO-1 and possible colocalization with PGP9.5 and DAPI in duodenum from WKY, vehicle-treated SHR, and ROSI- treated SHR rats. Representatives photomicrographs of duodenum sections immunostained with antibodies against HO-1 (C, G, K), PGP9.5 (D, H, L), DAPI (E, I, M), and superimposed images (F, J, N). Visualization by inverted microscopy shows: (first row) immunoreactivity red fluorescence-associated staining for HO-1 in nerve cell bodies of myenteric plexus between the circular (cm) and the longitudinal muscle (lm) layers in duodenum sections from WKY (C), vehicle-treated SHR (G), and ROSI-treated SHR rats (K); second row shows green fluorescence-associated staining for PGP9.5, neuronal marker, in duodenum section from WKY (D), vehicle-treated SHR (H), and ROSI-treated SHR rats (L); third row shows blue fluorescence-associated staining for DAPI, nuclear marker, in duodenum section from WKY (E), vehicle-treated SHR (I), and ROSI-treated SHR rats (M); fourth row shows merge of green, red, and blue fluorescence indicative of co-localization for HO-1, PGP9.5 (yellow fluorescence), and DAPI in WKY (F), vehicle-treated SHR (J), and ROSI-treated SHR rats (N). ×630 magnification.

FilenameFormatSizeDescription
NMO_1798_sm_FigS1.TIF134KSupporting info item
NMO_1798_sm_FigS2.tif285KSupporting info item

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