Myenteric neural network activated by motilin in the stomach of Suncus murinus (house musk shrew)

Authors

  • A. Mondal,

    1. Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan
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  • Y. Kawamoto,

    1. Division of Genetics, Department of Cancer Biology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan
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  • T. Yanaka,

    1. Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan
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  • C. Tsutsui,

    1. Department of Advanced Research Laboratories, Nanocarbon Biodevice Research Center, Tokyo City University, Tokyo, Japan
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  • I. Sakata,

    1. Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan
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  • S.-I. Oda,

    1. Laboratory of Animal Management & Resources, Department of Zoology, Faculty of Science, Okayama University of Science, Kita-ku, Okayama, Japan
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  • T. Tanaka,

    1. Faculty of Pharmaceutical Sciences, Josai University, Sakado-shi, Saitama, Japan
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  • T. Sakai

    1. Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan
    2. Saitama University Brain Science Institute, Saitama University, Sakura-ku, Saitama, Japan
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Address for Correspondence
Takafumi Sakai, Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakura-ku, Saitama 338-8570, Japan.
Tel: +81-48-858-3869; fax: +81-48-858-3422;
e-mail: tsakai@mail.saitama-u.ac.jp

Abstract

Background  It has been shown in human and canine studies that motilin, a gastroprokinetic hormone, induces gastric phase III contractions via the enteric nervous; however, the center of motilin action in the stomach has not been clearly revealed. In the present study, we investigated the neural pathway of motilin-induced gastric contraction by using Suncus murinus, a new animal model for motilin study.

Methods  An isolated suncus stomach was used in vitro to determine the mechanism of motilin action through the myenteric plexus. Synthetic suncus motilin (10−11–10−7 mol L−1) was added to an organ bath, and the spontaneous contraction response was expressed as a percent of ACh (10−5 mol L−1) responses. Motilin-induced contractions were also studied by a pharmacological method using several receptor antagonists and enzyme inhibitor.

Key Results  Suncus motilin induced a concentration-dependent gastric contraction at concentrations from 10−9 to 10−7 mol L−1. The responses to suncus motilin in the stomach were completely abolished by atropine and tetrodotoxin treatment and significantly suppressed by administration of hexamethonium, verapamil, phentolamine, yohimbine, ondansetron, and naloxone, whereas ritanserin, prazosin, timolol, and FK888 did not affect the action of motilin. Additionally, N-nitro l-arginine methylester slightly potentiated the contractions induced by motilin.

Conclusions & Inferences  The results indicate that motilin directly stimulates and modulates suncus gastric contraction through cholinergic, adrenergic, serotonergic, opioidergic, and NO neurons in the myenteric plexus.

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