5-HT3 and 5-HT4 receptors contribute to the anti-motility effects of Garcinia buchananii bark extract in the guinea-pig distal colon

Authors


Address for Correspondence
Onesmo B. Balemba, MVM, PhD, 252 Life Science Building, University of Idaho, Moscow, ID 83844-3051, USA.
Tel: +01 (208) 885 8023; fax: +01 (208) 885 7905;
e-mail: obalemba@uidaho.edu

Abstract

Background Garcinia buchananii bark extract is an anti-motility diarrhea remedy. We investigated whether G. buchananii bark extract has components that reduce gastrointestinal peristaltic activity via 5-HT3 and 5-HT4 receptors.

Methods  Aqueous G. buchananii extract was separated into fractions using preparative thin layer chromatography (PTLC), and major chemical components were identified using standard tests. The anti-motility effects of the extract and its fractions (PTLC1-5) were studied through pellet propulsion assays using isolated guinea-pig distal colons.

Key Results  Anti-motility (PTLC1 & PTLC5) and pro-motility (PTLC2) fractions were isolated from the extract. Flavonoids, steroids, alkaloids, tannins, and phenols were identified in the extract and PTLC1&5. The potency of the extract applied via the mucosal surface was reduced by 5-HT, 5-HT3 receptor agonist RS-56812, 5-HT4 receptor agonists cisapride and CJ-033466, 5-HT3 receptor antagonist granisetron, and 5-HT4 receptor antagonist GR-113808. The anti-motility effects of the aqueous extract and PTLC1&5 when applied serosally were reversed by RS-56812, cisapride, and CJ-033466. The 5-HT3 receptor antagonists, granisetron and ondansetron, reduced the effects of the extract to an extent and completely reversed the anti-motility effects of PTLC1&5. GR-113808 inhibited the actions of the extract during the initial 10 min, but enhanced the extracts’ anti-motility effects after 15 min. GR-113808 augmented the anti-motility activities of PTLC1 and PTLC5 by 30%.

Conclusions & Inferences  These results indicate that the anti-motility effects of G. buchananii aqueous extract are potentially mediated by compounds that affect 5-HT3 and 5-HT4 receptors. Identification and characterization of the bioactive compounds within G. buchananii could lead to the discovery of new non-opiate anti-diarrhea formulations.

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