Evidence of oligodendrogliosis in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonism

Authors

  • V. Annese,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
    3. Istituto Pasteur-Fondazione Cenci Bolognetti, Dip. di Biologia e Biotecnologie ‘Charles Darwin’
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    • Valentina Annese and Carlos Barcia contributed equally to this work.

  • C. Barcia,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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    • Valentina Annese and Carlos Barcia contributed equally to this work.

  • F. Ros-Bernal,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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  • A. Gómez,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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  • C. M. Ros,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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  • V. De Pablos,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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  • E. Fernández-Villalba,

    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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  • M. E. De Stefano,

    Corresponding author
    1. Istituto Pasteur-Fondazione Cenci Bolognetti, Dip. di Biologia e Biotecnologie ‘Charles Darwin’
    2. Center for Research in Neurobiology ‘Daniel Bovet’, Sapienza Università di Roma, P.le Aldo Moro, Roma, Italy
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  • M.-T. Herrero

    Corresponding author
    1. Clinical and Experimental Neuroscience
    2. Centro de Investigación Biomedica en Red de Enfermedades Neurodegenerativas (CIBERNED), School of Medicine, University of Murcia, Campus de Espinardo, Murcia, Spain
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María-Trinidad Herrero, Clinical and Experimental Neuroscience (NiCE-CIBERNED), School of Medicine, Campus de Espinardo, University of Murcia, 30071 Murcia, Spain. Tel: +34 868 88 46 83/84 84; Fax: +34 868 88 41 50; E-mail: mtherrer@um.es; M. Egle De Stefano, Dip. di Biologia e Biotecnologie ‘Charles Darwin’, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Roma, Italy. Tel: +39 06 4991 2230; Fax: +39 06 4991 2351; E-mail: egle.destefano@uniroma1.it

Abstract

V. Annese, C. Barcia, F. Ros-Bernal, A. Gómez, C. M. Ros, V. De Pablos, E. Fernández-Villalba, M.-E. De Stefano and M. T. Herrero (2013) Neuropathology and Applied Neurobiology39, 132–143

Evidence of oligodendrogliosis in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonism

Aims: Mice and nonhuman primates administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) represent elective experimental models of Parkinsonism, in which degeneration of the nigrostriatal dopaminergic pathway is associated with prominent neuroinflammation, characterized by activated microglia and astrocytes in both substantia nigra (SN) and striatum. To date, it is unknown whether oligodendrocytes play a role in these events. Methods: We performed a detailed qualitative and quantitative analysis of oligodendrocyte-associated changes induced by acute and chronic MPTP treatment, in the SN and striatum of mice and macaques respectively. Oligodendrocytes were immunolabelled by cell-specific markers and analysed by confocal microscopy. Results: In both experimental models, MPTP treatment induces an increase in oligodendrocyte cell number and average size, as well as in the total area occupied by this cell type per tissue section, accompanied by evident morphological changes. This multifaceted array of changes, herein referred to as oligodendrogliosis, significantly correlates with the reduction in the level of dopaminergic innervation to the striatum. Conclusions: This event, associated with early damage of the dopaminergic neurone axons and of the complex striatal circuits of which they are part, may result in an important, although neglected, aspect in the onset and progression of Parkinsonism.

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